Induction of Apoptosis in HeLa by Corn Small RNAs

Nutrition and Cancer
Mamta ShekhawatNilesh Kumar Sharma

Abstract

Insights in RNA biology have opened up a plethora of opportunities to explore the small regulatory RNAs from various natural and artificial sources. These small RNAs have been suggested to play a role too in tumor progression by either as oncogenic or tumor suppressor small RNAs. In this study, authors have attempted to evaluate the therapeutic potential of small RNAs fractionated from corn (Zea mays) upon growth and survival of HeLa. Here, authors have employed standard cellular-based approaches including microscopy, spectroscopy, and flow cytometry-based staining assays. Our data indicate that corn small RNAs fraction can appreciably decrease HeLa cell proliferation and survival, which is supported by a number of complementary assays such as Trypan blue dye exclusion, MTT, propidium iodide, and Annexin V/PI apoptotic cell death. Taken together, present finding suggests that corn small RNAs fraction may display up to 70% reduction in HeLa cell viability. Furthermore, these data indicate that around 40-50% of HeLa cells become apoptotic due to exogenous use of corn small. Overall, this finding proposes that possibility of cross-kingdom anticancer use of small RNAs from corn and present data need to be explored in depth.

References

Oct 27, 2001·Science·R C Lee, V Ambros
Dec 6, 2001·Nature Reviews. Genetics·S R Eddy
Jul 19, 2003·Science·James C Carrington, Victor Ambros
Jul 10, 2004·Cell·Haidi ZhangWitold Filipowicz
Oct 10, 2006·Oncogene·M Jovanovic, M O Hengartner
Nov 17, 2007·Cell Death and Differentiation·Andrew Z Fire
Feb 26, 2009·Cell·Richard W Carthew, Erik J Sontheimer
Jul 7, 2009·Annual Review of Cell and Developmental Biology·Xuemei Chen
Jul 10, 2010·Nucleic Acids Research·Kai WangDavid J Galas
Feb 23, 2012·BioEssays : News and Reviews in Molecular, Cellular and Developmental Biology·Mengxi JiangZhi Hong
Mar 16, 2012·Current Opinion in Lipidology·Kasey C Vickers, Alan T Remaley
Mar 19, 2013·Oncogene·D T VereideB Sugden
Apr 5, 2013·Immunological Reviews·Marshall P Thomas, Judy Lieberman
Apr 30, 2014·Nature Reviews. Genetics·Kevin V Morris, John S Mattick
Feb 24, 2015·The Journal of Nutritional Biochemistry·Hongwei LiangChen-Yu Zhang
Feb 28, 2015·Cell Research·Jian YangKendal D Hirschi
Feb 28, 2015·Cell Research·Sizolwenkosi MlotshwaVicki Vance
Mar 31, 2015·Journal of Extracellular Vesicles·Erez EitanMark P Mattson
Jun 27, 2015·Trends in Biotechnology·Kendal D HirschiVicki Vance
Jan 23, 2016·Cell Research·Andrew R ChinShizhen Emily Wang
May 12, 2016·Scientific Reports·Duccio CavalieriRoberto Viola
Sep 9, 2016·Scientific Reports·Chiara PastrelloIgor Jurisica
Apr 7, 2017·Scientific Reports·Yi LuoMingzhou Li
Jun 8, 2017·International Journal of Molecular Sciences·Dinesh KumarJayashankar Das
Jul 12, 2017·Genes & Nutrition·Kenneth W Witwer, Chen-Yu Zhang
Jul 18, 2017·Molecular Medicine Reports·Jie LiJian Tang
Sep 9, 2017·Cancer Biology & Medicine·Sunny YadavNilesh Kumar Sharma
Oct 13, 2017·Molecular Oncology·Recep BayraktarGeorge A Calin
Feb 8, 2018·Journal of Extracellular Vesicles·Roman KornilovSippy Kaur

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Citations

Oct 9, 2019·International Journal of Biological Macromolecules·Kai-Yue CaoZhi-Hong Jiang
Oct 22, 2020·Seminars in Cancer Biology·Alberto DávalosDavid de Gonzalo-Calvo

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Methods Mentioned

BETA
transfection
electrophoresis
electrophoresing
Assay
ELISA
FACS
transfections

Related Concepts

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Apoptosis

Apoptosis is a specific process that leads to programmed cell death through the activation of an evolutionary conserved intracellular pathway leading to pathognomic cellular changes distinct from cellular necrosis