Induction of apoptosis in HT-29 cells infected with SA-11 rotavirus

Journal of Medical Virology
F SupertiG Donelli

Abstract

Rotavirus infection is associated both in vivo and in vitro with a series of subcellular pathological alterations leading to cell lysis. It has been suggested that these modifications can play a key role in the pathogenesis of rotavirus-associated diarrheal disease. We describe the effects of SA-11 rotavirus infection in HT-29 cells, a human enterocyte-like cell line. Cytological analyses suggested that the viral-induced cytopathic process, including chromatin clumping, can be referred to as apoptosis, the cell death pathway alternative to necrosis. A time course of the process was performed to investigate whether rotavirus-associated cell death showed specific injury signs. HT-29-infected cells were analyzed by scanning and transmission electron microscopy and features of apoptosis such as blebbing of the plasma membrane, peripheral condensation of chromatin, and fragmentation of the nucleus were observed. Specific changes occurring in cell-substrate adhesion and in some organelles relevant for viral maturation, i.e., rough endoplasmic reticulum, were detected. These findings indicate a role for apoptosis in the rotavirus infection process and its related cytopathology, and also suggested that specific histological alterations...Continue Reading

References

Jul 10, 1992·Science·L MeyaardF Miedema
Jan 1, 1992·Annual Review of Pharmacology and Toxicology·P NicoteraS Orrenius
Jun 1, 1992·Trends in Pharmacological Sciences·W BurschR Schulte-Hermann
Jul 25, 1991·The New England Journal of Medicine·N R Blacklow, H B Greenberg
Apr 1, 1991·Immunology Today·J C Ameisen, A Capron
Jan 1, 1991·Archives of Virology·F SupertiG Donelli
Jan 1, 1991·Advances in Immunology·J J Cohen
May 1, 1987·Virology·M S ShahrabadiP W Lee
Jan 1, 1988·The American Journal of Cardiology·H KelbaekO Munck
Jun 1, 1995·Journal of Virology·L M EsolenD E Griffin
Feb 1, 1995·Experimental Cell Research·L M Desjardins, J P MacManus
Feb 15, 1995·Biochemical and Biophysical Research Communications·W MalorniC Franceschi
Jul 1, 1994·The Journal of General Virology·A C Vasconcelos, K M Lam
Jul 15, 1994·Biochemical and Biophysical Research Communications·B ZhivotovskyS Orrenius
Jun 1, 1993·Trends in Microbiology·A Zychlinsky
May 20, 1994·Cell·E Ruoslahti, J C Reed
Jun 1, 1994·Journal of Virology·V S HinshawD Evans
May 1, 1993·Cell Biology International·V G Evans

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Citations

Feb 16, 2007·Journal of Virology·Sandra Martin-LatilBruno Blondel
Oct 3, 2015·Tumour Biology : the Journal of the International Society for Oncodevelopmental Biology and Medicine·Zahra GoodarziEsmaeil Saberfar
Jan 10, 2013·Critical Reviews in Microbiology·Andre G Buret, Amol Bhargava
Jul 27, 2004·Memórias do Instituto Oswaldo Cruz·Juliana Galera CastilhoCarlos Nozawa
May 1, 2007·Apoptosis : an International Journal on Programmed Cell Death·Dirk Eulitz, Hans Georg Mannherz
Feb 13, 2008·Scandinavian Journal of Gastroenterology·Anthony G Catto-SmithRuth F Bishop
Sep 26, 2015·The Journal of General Virology·Gavan HollowayBarbara S Coulson
Mar 29, 2013·The Journal of Immunology : Official Journal of the American Association of Immunologists·Anastasia N VlasovaLinda J Saif
Feb 1, 2005·Virology·Charlotte ChaïbiMonique Géniteau-Legendre
Apr 21, 2010·Journal of Virological Methods·Carlos A GuerreroOrlando Acosta

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