Induction of apoptosis in human acute leukemia Jurkat T cells by Albizzia julibrissin extract is mediated via mitochondria-dependent caspase-3 activation

Journal of Ethnopharmacology
Hae Jeung WonKwang-Hyeon Kim

Abstract

To understand antitumor activity of Albizzia julibrissin Durazz (Leguminosae), which has been used as a traditional oriental medicine, the mechanism underlying cytotoxic effect of its extract on human acute leukemia Jurkat T cells were investigated. The methanol extract of the stripped barks (3kg) of Albizzia julibrissin was evaporated, dissolved in water, and then sequentially extracted by chloroform, ethyl acetate, and n-butanol. The substance in the butanol extract containing the most cytotoxic activity was further purified by a series of preparative column chromatography. The active substance obtained (723mg) was designated as HaBC18. When Jurkat T cells were treated with HaBC18 (0.5-2microg/ml), apoptosis along with several biochemical events such as mitochondrial cytochrome c release, activation of caspase-9 and -3, degradation of PARP, and DNA fragmentation was induced in a dose-dependent manner. However, the HaBC18-induced apoptosis was abrogated by an ectopic overexpression of Bcl-xL, which is known to block mitochondrial cytochrome c release. Primary cultures of human PBMC were less sensitive to the cytotoxicity relative to Jurkat T cells. These results demonstrate that the cytotoxicity of HaBC18 toward Jurkat T cells...Continue Reading

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Citations

May 28, 2010·Journal of Genetics·Subbashree Aparajita, Gyana Ranjan Rout
Aug 16, 2013·International Journal of Nanomedicine·Heshu Sulaiman RahmanSwee Keong Yeap
Sep 25, 2009·World Journal of Gastroenterology : WJG·Zong-Fang LiXian-Ming Xia
Feb 11, 2016·Complementary Therapies in Medicine·Hyun-Mok JuKi Han Kwon
Mar 13, 2015·Biological Research·Nguyen Phuong ThaoChau Van Minh
Nov 10, 2012·Experimental and Therapeutic Medicine·Minghe LinDezeng Zhu

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