PMID: 8940662Aug 1, 1996Paper

Induction of apoptotic cell death in the seminiferous tubule of the adult rat testis: assessment of the germ cell types that exhibit the ability to enter apoptosis after hormone suppression by oestradiol treatment

International Journal of Andrology
J Blanco-Rodríguez, C Martínez-García

Abstract

The involvement of apoptosis in germ cell degeneration in the adult rat testis after gonadotrophin suppression has recently been shown in several studies, which have focused on the localization of apoptotic cells to the stages of the spermatogenic cycle. However, the precise germ cell types and maturing steps at which apoptosis is elicited remain controversial. The present study used oestradiol treatment to produce hormone suppression and to study induced germ cell degeneration. Adult male rats were administered a daily injection of 50 micrograms oestradiol benzoate for 5, 10 or 15 days. Characterization of the ultrastructural features of the dying cells and in-situ 3'-end labelling of DNA showed clearly that the deaths of all the germ cell types occurred by apoptosis. High-resolution light microscopy, although time-consuming, resulted in a precise method for analysis of the localization of the cells involved. Stages IV-X of the seminiferous epithelium were found to be the most sensitive to degeneration in response to oestradiol treatment. Our results are discussed in the light of current knowledge about the hormonal control of the spermatogenic cycle. Oestradiol treatment has proved to provide a suitable in-vivo model to study...Continue Reading

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Citations

May 8, 2000·BioEssays : News and Reviews in Molecular, Cellular and Developmental Biology·C G Print, K L Loveland
Oct 14, 2003·Reproductive Toxicology·Alireza HossainiJens Jørgen Larsen
Jun 19, 2001·Molecular and Cellular Endocrinology·Y ToyamaS Yuasa
Apr 12, 2003·Anatomical Science International·Yoshiro ToyamaShigeki Yuasa
Mar 17, 1999·Aging : Clinical and Experimental Research·C J BarnesM Lee
Jun 30, 2004·Human Fertility : Journal of the British Fertility Society·Denny SakkasDavide Bizzaro
Jun 30, 2004·The Anatomical Record. Part A, Discoveries in Molecular, Cellular, and Evolutionary Biology·Taiza StumppSandra Maria Miraglia
Apr 11, 2008·The Journal of Steroid Biochemistry and Molecular Biology·Nikolaos SofikitisNikolaos Pardalidis
Jul 15, 2009·Journal of Cellular Physiology·Carlos LizamaRicardo D Moreno
Aug 11, 2015·Expert Reviews in Molecular Medicine·Sara CorreiaSílvia Socorro
Nov 28, 2006·Reproduction : the Official Journal of the Society for the Study of Fertility·A StronatiD Bizzaro
Nov 7, 1998·International Journal of Andrology·J Blanco-Rodríguez
May 1, 2014·Spermatogenesis·Caitlin J Murphy, John H Richburg
Oct 3, 2018·Frontiers in Endocrinology·Donatella PaoliFrancesco Lombardo

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Apoptosis

Apoptosis is a specific process that leads to programmed cell death through the activation of an evolutionary conserved intracellular pathway leading to pathognomic cellular changes distinct from cellular necrosis