PMID: 7033965Oct 1, 1981Paper

Induction of autoantibody-producing cells after the coculture of haptenated and normal human mononuclear leukocytes

Proceedings of the Society for Experimental Biology and Medicine
E J PiskoR A Turner

Abstract

The coculture of normal human peripheral blood mononuclear leukocytes (PBL) and autologous mononuclear leukocytes coupled to the trinitrophenyl (TNP) hapten (TNP-PBL) was found to induce a polyclonal activation of antibody-producing cells. The polyclonal activation of antibody-producing cells was demonstrated by detecting the induction of cells producing antibody to sheep red blood cells using a complement-dependent, direct, hemolytic plaque-forming cell (PFC) assay. A ratio of four normal to one haptenated mononuclear leukocyte was found to be optimal for inducing the polyclonal activation of antibody-producing cell in these cultures. The plaque-forming cells assay in these experiments utilized monolayers of indicator red cells. Further evidence for the polyclonal induction of antibody-producing cells by TNP-PBL was provided by demonstrating PFC on monolayers of not only sheep red blood cells, but also autologous human red cells, bromelain-treated autologous red cells, TNP-coupled human and sheep red cells, and human autologous red cells coupled to human heat-aggregated IgG with chromic chloride. Thus cells secreting antibody to TNP, human red cells, and human IgG were induced. Anti-IgG and anti-human red cell-producing cells ...Continue Reading

Citations

Jan 1, 1983·International Journal of Immunopharmacology·E J PiskoR A Turner
Jun 1, 1983·Annals of the Rheumatic Diseases·R A TurnerW J Treadway
Sep 1, 1982·Arthritis and Rheumatism·E J PiskoS L Foster

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