PMID: 9530995Apr 8, 1998Paper

Induction of axon-like and dendrite-like processes in neuroblastoma cells

Journal of Neurocytology
G WuRobert W Ledeen

Abstract

Neuroblastoma cells are widely utilized models for the study of the neuritic outgrowth phase of neuronal differentiation, but relatively few such studies have attempted to identify the nature of the process outgrowths. This identification will be necessary in developing strategies for utilizing these models to distinguish the underlying mechanisms involved in axonogenesis vs dendritogenesis. In an effort to identify procedures for inducing specific types of neurite outgrowth, and for distinguishing axon- from dendrite-like processes, we have subjected two neuroblastoma cell lines to a variety of stimuli previously shown to induce neurite outgrowth in these cells. These include neuraminidase, ionomycin, KCl+dibutyryl cAMP, cholera toxin B subunit, retinoic acid, dibutyryl cAMP (alone), GM1 ganglioside, and low serum. The first four of these (group 1) gave rise to neurites with axon-like characteristics, including immunostaining that was positive for phosphorylated high molecular weight neurofilament protein (NF-H) and synaptic vesicle protein-2 (SV2), but negative for microtubule-associated protein-2 (MAP2). The next three treatments (group 2) resulted in dendrite-like processes, as evidenced in immunostaining that was positive ...Continue Reading

Citations

Jun 19, 2004·Journal of Toxicology and Environmental Health. Part a·Taehyeon Cho, Evelyn Tiffany-Castiglioni
Aug 12, 2006·Canadian Journal of Physiology and Pharmacology·Robert W Ledeen, Gusheng Wu
Mar 1, 2008·Neurochemical Research·Kimberly A Petro, Cara-Lynne Schengrund
Dec 4, 2009·PLoS Pathogens·Sina GhaemmaghamiStanley B Prusiner
Oct 4, 2011·Molecular Biology of the Cell·Elif Nur Firat-KaralarMatthew D Welch
Dec 18, 2013·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·Nobuharu SuzukiChihiro Akazawa
Jul 21, 1998·Annals of the New York Academy of Sciences·R W LedeenY Fang
Aug 16, 2006·Molecular and Cellular Biochemistry·Ping-An Chang, Yi-Jun Wu
May 12, 2006·Journal of Neurobiology·Peter McCafferyJames E Crandall
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Sep 8, 2006·Environmental Health : a Global Access Science Source·Eiichi AkahoshiMitsuko Ishihara-Sugano
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Aug 19, 2009·Journal of Neurochemistry·Jianfeng WangRobert W Ledeen
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