Induction of BCR-ABL-specific immunity following vaccination with chaperone-rich cell lysates derived from BCR-ABL+ tumor cells.

Blood
Yi ZengEmmanuel Katsanis

Abstract

We have previously reported that chaperonerich cell lysates (CRCL) derived from the BCR-ABL+ 12B1 leukemia activate dendritic cells (DCs) and stimulate leukemia-specific immune responses. Because CRCL contain a variety of heat shock/chaperone proteins, we theorized that CRCL obtained from BCR-ABL+ leukemias are likely to chaperone BCR-ABL-derived fusion peptides and that DCs pulsed with 12B1 CRCL could cross-present BCR-ABL fusion peptides to T cells. We found that splenocytes from mice vaccinated with BCR-ABL+ leukemia-derived CRCL secreted interferon-gamma (IFN-gamma) when restimulated with a BCR-ABL peptide, GFKQSSKAL, indicating that BCR-ABL peptides are chaperoned by leukemia-derived CRCL. We next eluted peptides from 12B1 leukemia-derived CRCL and used high-pressure liquid chromatography (HPLC) fractions to restimulate splenocytes harvested from mice vaccinated with DC/GFKQSSKAL or DC/12B1 CRCL. We found that the same peptide fractions derived from 12B1 CRCL and from "refractionated" GFKQSSKAL stimulated IFN-gamma production, suggesting the presence of BCR-ABL peptides in the peptide repertoire of 12B1 CRCL. We also demonstrated that immunization with DCs loaded with leukemia-derived CRCL induced BCR-ABL-specific cytotoxi...Continue Reading

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Citations

Aug 2, 2005·Neuro-oncology·Michael W Graner, Darell D Bigner
Feb 18, 2010·Immunotherapy·Nona JanikashviliEmmanuel Katsanis
Jun 4, 2013·International Journal of Hyperthermia : the Official Journal of European Society for Hyperthermic Oncology, North American Hyperthermia Group·Michael W GranerEmmanuel Katsanis
Jul 9, 2008·International Journal of Hyperthermia : the Official Journal of European Society for Hyperthermic Oncology, North American Hyperthermia Group·Elke BleifussRolf-Dieter Issels
Feb 17, 2011·Expert Review of Hematology·Gheath Alatrash, Jeffrey J Molldrem
Mar 27, 2007·Clinical Lymphoma & Myeloma·Tariq Mughal, John M Goldman
Aug 16, 2005·British Journal of Haematology·Louise K Jones, Vaskar Saha
Sep 22, 2006·International Journal of Cancer. Journal International Du Cancer·Yi ZengEmmanuel Katsanis
Aug 8, 2006·Biotechnology Journal·Manfred SchusterRalf Kircheis
Mar 1, 2006·Biology of Blood and Marrow Transplantation : Journal of the American Society for Blood and Marrow Transplantation·Xinchun ChenEmmanuel Katsanis
Jan 22, 2015·Molecular & Cellular Proteomics : MCP·Yang YuQi Jin
May 12, 2005·Cancer Immunology, Immunotherapy : CII·Yi ZengEmmanuel Katsanis
Mar 1, 2007·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·Kerri L KislinEmmanuel Katsanis

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