PMID: 7536509Nov 1, 1994Paper

Induction of bleomycin resistance in a human oral squamous carcinoma cell line and characterisation of bleomycin-resistant and -sensitive clones

European Journal of Cancer. Part B, Oral Oncology
M UradeW Yoshioka

Abstract

We examined the change of sensitivity to antitumour agents by repeated treatment with bleomycin (BLM) using two oral squamous carcinoma cell lines, SCCTF and SCCKN. SCCTF exhibited minimal sensitivity to BLM and strong heterogeneity in BLM sensitivity, whereas SCCKN was highly sensitive to BLM and showed weak heterogeneity. When SCCTF was treated continuously with low-dose BLM (0.1 microgram/ml) but not intermittently with high-dose BLM (1 microgram/ml), the BLM sensitivity was rapidly decreased to acquire drug resistance. On the other hand, SCCKN was completely killed by the same treatments. To investigate the mechanism of induction of resistance in SCCTF, BLM-sensitive and -resistant clones, TF-S and TF-R, were isolated and analysed. Consequently, TF-R showed decreased cellular accumulation and retention of BLM, increased BLM hydrolase activity and elevated DNA repair activity concomitant with increased poly(ADP-ribose) polymerase activity as compared with TF-S. Therefore, it was suggested that antitumour drug-resistant clones were selectively grown from heterogeneous tumour cell population.

Citations

Apr 6, 2001·International Journal of Cancer. Journal International Du Cancer·T UematsuM Yamaoka
Sep 21, 2007·Journal of Cellular Biochemistry·Hirohiko OkamuraTatsuji Haneji
Feb 10, 2006·Annals of Botany·Christos KatsarosBasil Galatis
May 22, 2007·The Journal of Immunology : Official Journal of the American Association of Immunologists·Charles F TowneKenneth L Rock

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