Induction of blood brain barrier tight junction protein alterations by CD8 T cells.

PloS One
Georgette L SuidanAaron J Johnson

Abstract

Disruption of the blood brain barrier (BBB) is a hallmark feature of immune-mediated neurological disorders as diverse as viral hemorrhagic fevers, cerebral malaria and acute hemorrhagic leukoencephalitis. Although current models hypothesize that immune cells promote vascular permeability in human disease, the role CD8 T cells play in BBB breakdown remains poorly defined. Our laboratory has developed a novel murine model of CD8 T cell mediated central nervous system (CNS) vascular permeability using a variation of the Theiler's virus model of multiple sclerosis. In previous studies, we observed that MHC class II(-/-) (CD4 T cell deficient), IFN-gammaR(-/-), TNF-alpha(-/-), TNFR1(-/-), TNFR2(-/-), and TNFR1/TNFR2 double knockout mice as well as those with inhibition of IL-1 and LTbeta activity were susceptible to CNS vascular permeability. Therefore, the objective of this study was to determine the extent immune effector proteins utilized by CD8 T cells, perforin and FasL, contributed to CNS vascular permeability. Using techniques such as fluorescent activated cell sorting (FACS), T1 gadolinium-enhanced magnetic resonance imaging (MRI), FITC-albumin leakage assays, microvessel isolation, western blotting and immunofluorescent mi...Continue Reading

References

Aug 1, 1991·Journal of Neuro-oncology·D L Silbergeld, F Ali-Osman
Nov 13, 1998·Genes to Cells : Devoted to Molecular & Cellular Mechanisms·S Tsukita, M Furuse
Sep 4, 1999·Neuropathology and Applied Neurobiology·H BrownG Turner
Dec 5, 2000·Molecular Biology of the Cell·M SaitouS Tsukita
Feb 17, 2001·American Journal of Physiology. Heart and Circulatory Physiology·J D HuberT P Davis
Oct 19, 2001·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·A J JohnsonL R Pease
May 23, 2002·The Journal of Immunology : Official Journal of the American Association of Immunologists·Iva ZivnaAlan L Rothman
Sep 18, 2002·American Journal of Physiology. Heart and Circulatory Physiology·J D HuberT P Davis
May 14, 2003·The Journal of Cell Biology·Takehiro NittaShoichiro Tsukita
Jun 17, 2003·Nature Medicine·Juthathip MongkolsapayaGavin Screaton
Aug 9, 2003·American Journal of Physiology. Heart and Circulatory Physiology·Ken A WittThomas P Davis
Feb 24, 2004·The Journal of Immunology : Official Journal of the American Association of Immunologists·Elizabeth D KilpatrickFrancis A Ennis
Jun 23, 2004·Neurobiology of Disease·Praveen BallabhMaiken Nedergaard
Jul 30, 2004·The Journal of Neuroscience : the Official Journal of the Society for Neuroscience·Matteo MartiMichele Morari
Mar 29, 2005·American Journal of Physiology. Heart and Circulatory Physiology·Tracy A BrooksThomas P Davis
May 21, 2005·The Journal of Immunology : Official Journal of the American Association of Immunologists·Aaron J JohnsonLarry R Pease
May 26, 2005·Pharmacological Reviews·Brian T Hawkins, Thomas P Davis
Sep 30, 2005·Journal of Cerebral Blood Flow and Metabolism : Official Journal of the International Society of Cerebral Blood Flow and Metabolism·Oliver B DimitrijevicAnuska V Andjelkovic
Feb 28, 2006·International Journal for Parasitology·Sarah PotterNicholas H Hunt
Apr 18, 2006·International Journal for Parasitology·Isabelle M Medana, Gareth D H Turner
May 12, 2006·Neurological Research·Georgette L SuidanAaron J Johnson
May 12, 2006·Neurological Research·Jeremiah McDoleIstvan Pirko
Sep 9, 2006·European Journal of Cell Biology·Ruud D FontijnAnton J G Horrevoets
Sep 26, 2006·Journal of Cellular Physiology·Sumio OhtsukiTetsuya Terasaki
Aug 8, 2007·The Journal of Experimental Medicine·Ian GaleaV Hugh Perry

❮ Previous
Next ❯

Citations

Mar 30, 2013·The International Journal of Biochemistry & Cell Biology·Romy RazakandrainibeRonan Jambou
Jul 3, 2013·International Journal of Inflammation·Kate Marie LewisRobert Vink
Aug 27, 2014·Mediators of Inflammation·Sin Yee GunLaurent Rénia
Oct 6, 2009·Seminars in Immunopathology·Bettina SchreinerBurkhard Becher
May 5, 2010·The Journal of Clinical Investigation·Emma H WilsonChristopher A Hunter
Dec 30, 2010·Molecular Therapy : the Journal of the American Society of Gene Therapy·Xi ZhaoWalter J Storkus
Feb 19, 2011·Nature Reviews. Neuroscience·Edward A NeuweltLester R Drewes
Feb 23, 2011·Antioxidants & Redox Signaling·Caroline Coisne, Britta Engelhardt
Nov 25, 2011·Journal of Neuroinflammation·Ganta V ChaitanyaJ Steven Alexander
Dec 9, 2010·European Journal of Clinical Microbiology & Infectious Diseases : Official Publication of the European Society of Clinical Microbiology·S Balachandar, A Katyal
Apr 20, 2010·Journal of Neuroimmune Pharmacology : the Official Journal of the Society on NeuroImmune Pharmacology·Silvia S Kang, Dorian B McGavern
May 10, 2011·FEBS Letters·Catherine LarochelleAlexandre Prat
May 9, 2012·Trends in Microbiology·Katherine R Spindler, Tien-Huei Hsu
Sep 4, 2010·The American Journal of Pathology·Jeremiah R McDoleAaron J Johnson
Nov 15, 2011·The American Journal of Pathology·Norinne Lacerda-QueirozAntonio L Teixeira
Sep 16, 2010·Journal of Cerebral Blood Flow and Metabolism : Official Journal of the International Society of Cerebral Blood Flow and Metabolism·Luca CuculloDamir Janigro
Nov 2, 2013·Scientific Reports·Hayder JafferVinod Labhasetwar
May 2, 2013·Parasite Immunology·U Frevert, A Nacer
Jan 13, 2011·The American Journal of Pathology·Mathilde FauconnierValerie F J Quesniaux
Apr 4, 2015·Tissue Barriers·Stephen F Rodrigues, D Neil Granger
Mar 27, 2010·FEMS Microbiology Reviews·Hany M Elsheikha, Naveed Ahmed Khan
Apr 14, 2009·Veterinary Parasitology·G D MeloG F Machado
Mar 31, 2012·Virulence·Laurent RéniaLisa F P Ng
Aug 28, 2013·Medical Hypotheses·Yuri Chaves Martins, Cláudio Tadeu Daniel-Ribeiro
Jan 29, 2014·Fluids and Barriers of the CNS·Manuela Polimeni, Mauro Prato
Feb 1, 2017·Biochemical and Biophysical Research Communications·Su-Ping PengYan-Qin Shen
Sep 25, 2014·The Journal of Histochemistry and Cytochemistry : Official Journal of the Histochemistry Society·Rachel E MellasOlga J Baker
Oct 5, 2017·Archivum Immunologiae Et Therapiae Experimentalis·Katarzyna Donskow-ŁysoniewskaMaria Doligalska
Jun 29, 2013·International Journal of Psychiatry in Medicine·Fang-Yuan KoLie-Ming Xu

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Methods Mentioned

BETA
protein assay

Software Mentioned

SYSTAT
BD FACS Diva
SigmaPlot
Metamorph
SigmaStat
Scion Image
SpectraMax

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