Induction of bovine bronchial epithelial cell filopodia by tetradecanoyl phorbol acetate, calcium ionophore, and lysophosphatidic acid

Journal of Cellular Physiology
J D BeckmannJ R Spurzem

Abstract

The morphological responses of primary bovine bronchial epithelial cells (BBECs) cultured in serum-free medium to protein activators have been examined. When attached to type I collagen-coated tissue culture dishes, the cells responded to tetradecanoyl phorbol acetate (TPA), calcium ionophore A23187, and lysophosphatidic acid (LPA) by extruding filopodia. In contrast, no morphological changes were elicited by exposures to either epinephrine or dibutyryl-cAMP. Formation of filopodia was accompanied by actin filament reorganization as demonstrated by staining with labeled phalloidin. Exposures to varied TPA concentrations for 2 h showed maximal stimulation of filopodial extrusions at 10 nM TPA with half-maximal stimulation at 1 nM. Time-course measurements with 10 nM TPA showed filopodia formation within 30 min of exposure, with 85% of the BBECs being filopodia positive after 5 h. Filopodia induction in 20-30% of the cells could be achieved by 1-100 microM LPA concentrations. BBECs acquired increasing resistance to TPA-induced filopodia during the initial 5 days in culture; however, responsiveness to TPA was regenerated by mild treatment with trypsin. Inclusion of fibronectin or vitronectin into the attachment matrix had no effec...Continue Reading

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Citations

Oct 13, 2001·Journal of Cellular Physiology·J D BeckmannT P Keeton
Oct 15, 2003·The Journal of Cell Biology·Masaki ShigetaKiyotoshi Sekiguchi
Dec 15, 2015·Tumour Biology : the Journal of the International Society for Oncodevelopmental Biology and Medicine·Young Sun HwangPaul F Lindholm
May 27, 2006·Prostaglandins, Leukotrienes, and Essential Fatty Acids·Elizabeth BarekziJohn W Steinke
Jun 30, 2000·Pulmonary Pharmacology & Therapeutics·K RackéU R Juergens
Jul 21, 2007·The Journal of Pharmacology and Experimental Therapeutics·Karen M KasselMyron L Toews
Oct 24, 1997·Journal of Applied Physiology·M L ToewsH D Schultz

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