Induction of c-fos messenger ribonucleic acid in neuropeptide Y and growth hormone (GH)-releasing factor neurons in the rat arcuate nucleus following systemic injection of the GH secretagogue, GH-releasing peptide-6

Endocrinology
S L Dickson, S M Luckman

Abstract

In this study we investigated the neurochemical identity of the arcuate cells activated following GH-releasing peptide-6 (GHRP-6) injection by comparing, on consecutive sections, the distribution c-fos messenger RNA (mRNA) with that of mRNAs for peptides synthesized in arcuate cells, including neuropeptide Y (NPY), GH-releasing factor (GRF), tyrosine hydroxylase, POMC, and somatostatin. Rats bearing chronically implanted jugular catheters were injected with either 50 micrograms GHRP-6 or vehicle. Thirty minutes later they were terminally anesthetized and perfused with fixative. Paraffin-embedded sections of 7 microns thickness were processed using in situ hybridization for either c-fos mRNA or mRNAs for the neurochemical markers. In GHRP-6-treated rats the mean (+/-SEM) number of cells expressing c-fos mRNA in the arcuate nucleus (23 +/- 2 cells/section per rat; n = 5) was significantly higher than for vehicle-treated controls (2 +/- 1 cells/section per rat; n = 5; P < 0.001, Mann-Whitney U test). Superimposed camera lucida maps indicated that, in GHRP-6-injected rats, neurochemically identifiable cells expressing c-fos mRNA also express NPY mRNA (51 +/- 4%), GRF mRNA (23 +/- 1%) tyrosine hydroxylase mRNA (11 +/- 3%), POMC mRNA...Continue Reading

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Citations

Oct 14, 2008·Genes & Nutrition·L MaciaI Wolowczuk
Feb 23, 2011·Reviews in Endocrine & Metabolic Disorders·Emil EgeciogluSuzanne L Dickson
Jan 9, 2013·Proceedings of the National Academy of Sciences of the United States of America·Marie SchaefferPatrice Mollard
Oct 1, 2009·American Journal of Physiology. Endocrinology and Metabolism·Weizhen ZhangMichael W Mulholland
Nov 20, 2008·American Journal of Physiology. Endocrinology and Metabolism·Kimberly P KinzigErin E Tao
Aug 20, 2010·International Journal of Peptides·Wai W Cheung, Robert H Mak
Aug 28, 2010·International Journal of Peptides·Mark Fry, Alastair V Ferguson
Aug 28, 2010·International Journal of Peptides·Johannes D Veldhuis, Cyril Y Bowers
Jun 5, 2002·The Journal of Clinical Investigation·Yujin ShutoIchiji Wakabayashi
Jul 11, 2009·Endocrine Reviews·Claes OhlssonJohan Svensson
Feb 18, 2010·PloS One·Guillaume OsterstockPierre-François Méry
May 4, 2011·World Journal of Diabetes·Undurti N Das
Apr 9, 2013·Indian Journal of Endocrinology and Metabolism·Alfonso Abizaid, Tamas L Horvath
Feb 13, 2001·Biochemical and Biophysical Research Communications·S LallS L Dickson
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Nov 5, 2003·Gastroenterology·Charles D R MurrayAnton V Emmanuel
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Jul 1, 2009·Molecular and Cellular Endocrinology·Efthimia Karra, Rachel L Batterham
Apr 10, 2009·European Journal of Pharmacology·Nicolas SaloméSuzanne L Dickson
Jul 16, 2015·The Journal of Physiology·Gintautas GrabauskasChung Owyang
Oct 16, 2007·Regulatory Peptides·Emil EgeciogluSuzanne L Dickson
May 15, 2007·Gastroenterology·A M Wren, S R Bloom
Jun 5, 2015·Molecular Metabolism·T D MüllerM H Tschöp

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