Induction of CD8+ T-lymphocyte responses to a secreted antigen of Mycobacterium tuberculosis by an attenuated vaccinia virus

Immunology and Cell Biology
C G FengW J Britton

Abstract

Protective immunity against Mycobacterium tuberculosis infection requires the activation of mycobacterium-specific CD8+ T cells, as well as CD4+ T cells. Therefore, optimizing strategies that stimulate CD8+ T cells recognizing dominant mycobacterial antigens, including secreted proteins, may lead to the development of more effective vaccines against tuberculosis. To generate a viral vaccine that is safe in humans, the early secreted protein, MPT64, was expressed in the attenuated vaccinia virus (VV) strain, modified vaccinia virus Ankara (MVA-64). The immunogenicity of MVA-64 was compared with that of the Western Reserve strain of VV (VVWR-64). The replication-defective MVA-64 was as efficient as VVWR-64 in inducing specific antibodies and cytolytic T-cell responses to a defined H-2-Db-restricted epitope on MTP-64. In addition, priming with MPT64-expressing plasmid DNA (DNA-64), and boosting with either MVA-64 or VVWR-64, markedly enhanced MPT64-specific cytolytic and IFN-gamma-producing CD8+ T-cell responses. These findings suggest that MVA may be a suitable vaccine carrier for stimulating mycobacterium-specific CD8+ T-cell responses and may be particularly relevant for developing vaccines for use in regions endemic for tuberc...Continue Reading

References

Nov 15, 1992·Proceedings of the National Academy of Sciences of the United States of America·G Sutter, B Moss
Jan 1, 1992·Clinical and Experimental Immunology·R D HubbardF M Collins
Jun 6, 1991·The New England Journal of Medicine·P F BarnesD E Snider
Jan 1, 1990·Current Topics in Microbiology and Immunology·J R Bennink, J W Yewdell
Nov 27, 1969·The New England Journal of Medicine·J M LaneJ D Millar
Dec 1, 1993·The Journal of Experimental Medicine·A M CooperI M Orme
Mar 2, 1994·JAMA : the Journal of the American Medical Association·G A ColditzF Mosteller
Aug 1, 1993·Infection and Immunity·D McMahon-PrattN H Ruddle
Jul 1, 1993·American Journal of Clinical Pathology·C AbramowskyR U Sorensen
Aug 1, 1996·Nature Medicine·R E TasconD B Lowrie
Aug 1, 1996·Nature Medicine·K HuygenJ B Ulmer
Oct 15, 1996·Proceedings of the National Academy of Sciences of the United States of America·B Moss
Dec 21, 1996·Lancet·T BlanchardG L Smith
Dec 21, 1996·Lancet·R Arcieri, N Schinaia
Jun 1, 1997·Clinical Infectious Diseases : an Official Publication of the Infectious Diseases Society of America·E A TalbotR Frothingham
Jun 24, 1998·Proceedings of the National Academy of Sciences of the United States of America·M SedegahS L Hoffman
Nov 24, 1999·Immunological Reviews·J SchneiderA V Hill
Dec 3, 1999·Immunological Reviews·A J RamsayI A Ramshaw
Feb 29, 2000·Tubercle and Lung Disease : the Official Journal of the International Union Against Tuberculosis and Lung Disease·S L BaldwinJ B Ulmer
Apr 13, 2000·Proceedings of the National Academy of Sciences of the United States of America·A O SousaB R Bloom

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Citations

Jul 14, 2005·Nature Reviews. Microbiology·Peter Andersen, T Mark Doherty
Oct 16, 2002·American Journal of Respiratory and Critical Care Medicine·Vanja Lazarevic, JoAnne Flynn
Feb 6, 2009·The Journal of Gene Medicine·Kandan AravindaramNing-Sun Yang
May 10, 2005·Microbes and Infection·Peter Andersen, T Mark Doherty
Mar 29, 2016·Protein Expression and Purification·Hanyu ChenChenyun Guo
Jun 27, 2006·Microbes and Infection·Romulo AráozStewart T Cole
Jun 5, 2007·Expert Review of Vaccines·Samuel M BeharYing Wu
Jan 22, 2003·Immunology and Cell Biology·Warwick J Britton, Umaimainthan Palendira
Mar 25, 2011·Mucosal Immunology·J L FlynnP L Lin
Oct 24, 2006·The Journal of Immunology : Official Journal of the American Association of Immunologists·Arati KamathSamuel M Behar

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