Induction of CD83+CD14+ nondendritic antigen-presenting cells by exposure of monocytes to IFN-alpha

The Journal of Immunology : Official Journal of the American Association of Immunologists
Gianni GerliniNicola Pimpinelli

Abstract

IFN-alpha is a well-known agent for treatment of viral and malignant diseases. It has several modes of actions, including direct influence on the immune system. We investigated IFN-alpha effects on PBMC in terms of dendritic cell (DC) differentiation, as PBMC are exposed to high IFN-alpha levels during treatment of infections and cancers. We show that in vitro IFN-alpha exposure induced rapid and strong up-regulation of the DC-maturation markers CD80, CD86, and CD83 in bulk PBMC. Consistently, IFN-alpha induced up-regulation of these molecules on purified monocytes within 24 h. Up-regulation of CD80 and CD83 expression was IFN-alpha concentration-dependent. In contrast to GM-CSF + IL-4-generated DCs, most of the IFN-alpha-challenged CD83(+) cells coexpressed the monocyte marker CD14. Despite a typical mature DC immunophenotype, IFN-alpha-treated monocytes conserved phagocytic activity and never acquired a dendritic morphology. In mixed lymphocyte reactions IFN-alpha-treated monocytes were less potent than GM-CSF + IL-4-generated DCs but significantly more potent than untreated monocytes to induce T cell proliferation in bulk PBMC. However, only GM-CSF + IL-4-generated DCs were able to induce a significant proliferation of naive...Continue Reading

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Citations

Aug 4, 2009·American Journal of Physiology. Lung Cellular and Molecular Physiology·Jill A PooleDebra J Romberger
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May 7, 2010·Immunology and Cell Biology·Anthony L CunninghamStuart Turville
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Nov 28, 2020·Advanced Biosystems·Lili ZhouFangfang Zhou
Aug 17, 2021·Frontiers in Immunology·Alsya J AffandiJoke M M den Haan

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