Induction of cell cycle arrest and apoptosis by betulinic acid-rich fraction from Dillenia suffruticosa root in MCF-7 cells involved p53/p21 and mitochondrial signalling pathway

Journal of Ethnopharmacology
Jhi Biau FooRasedee Abdullah

Abstract

Dillenia suffruticosa (Family: Dilleniaceae) or commonly known as "Simpoh air" in Malaysia, is traditionally used for treatment of cancerous growth including breast cancer. D. suffruticosa root dichloromethane extract (DCM-DS) has been reported to induce G0/G1 phase cell cycle arrest and apoptosis in caspase-3 deficient MCF-7 breast cancer cells. The present study was designed to investigate the involvement of p53/p21 and mitochondrial pathway in DCM-DS-treated MCF-7 cells as well as to identify the bioactive compounds responsible for the cytotoxicity of DCM-DS. Extraction of D. suffruticosa root was performed by the use of sequential solvent procedure. GeXP-based multiplex system was employed to investigate the expression of p53, p21, Bax and Bcl-2 genes in MCF-7 cells treated with DCM-DS. The protein expression was then determined using Western blot analysis. The bioactive compounds present in DCM-DS were isolated by using column chromatography. The structure of the compounds was elucidated by using nuclear magnetic resonance spectroscopy. The cytotoxicity of the isolated compounds towards MCF-7 cells was evaluated by using MTT assay. The percentage of betulinic acid (BA) in DCM-DS was determined by HPLC analysis. The express...Continue Reading

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Citations

Nov 5, 2016·Biomedicine & Pharmacotherapy = Biomédecine & Pharmacothérapie·Runlan LuoZeyao Tang
Nov 5, 2016·Nanomedicine·Karina ValdésGermán Günther
Nov 28, 2016·Phytochemistry·Carla W SabandarNor-Ashila Aladdin
Jun 28, 2017·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Chunying LiWenyan Zhao
Mar 22, 2018·Artificial Cells, Nanomedicine, and Biotechnology·Fahmi YakopZolkapli Eshak
Jun 23, 2020·Journal of Toxicology and Environmental Health. Part a·Jurandy do Nascimento SilvaPaulo Michel Pinheiro Ferreira
Jan 25, 2020·Anti-cancer Agents in Medicinal Chemistry·Nádia M Garcês de CoutoSimone C B Gnoatto
Jun 6, 2019·International Journal of Molecular Sciences·Giuseppe Antonio MalfaClaudia Di Giacomo
Nov 13, 2020·Chemico-biological Interactions·Justyna KutkowskaAndrzej Rapak
Jan 29, 2021·Evidence-based Complementary and Alternative Medicine : ECAM·Muhammad Murtala MainasaraYazan K H Ranneh
Jun 3, 2021·International Journal of Molecular Sciences·Olakunle OladimejiMoganavelli Singh
May 2, 2018·Biomedicine & Pharmacotherapy = Biomédecine & Pharmacothérapie·Forouzan BahmaniAhmad Gharehbaghian

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