Induction of cell death in T lymphocytes by invasin via beta1-integrin

European Journal of Immunology
I ArencibiaKarl-Gösta Sundqvist

Abstract

Ligand binding to beta1-integrins exerts multiple effects on cells of the immune system including adhesion, spreading, haptotaxis and costimulation of T cells activated by anti-CD3. Here we show that a high-affinity ligand for beta1-integrins, the invasin (Inv) protein of Yersinia pseudotuberculosis, can induce cell death in T lymphocytes via a rapid process. Partially purified native Inv protein and an Inv fusion protein caused apoptotic/necrotic caspase-independent cell death in T lymphocytes as determined by phosphatidylserine exposure on the cell surface, uptake of propidium iodide, labeling of DNA strand breaks and presence of DNA ladder. Inv-induced cell death was mediated via beta1-integrins as indicated by the fact that Inv bound to the beta1-integrin subunit (CD29), that anti-beta(1)-integrin antibodies blocked Inv-induced cell death and that Inv-induced cell death was absent in two beta1-integrin- cell lines produced by different procedures. Killing via beta1-integrins represents a novel pathway for cell death in T lymphocytes.

References

Oct 1, 1991·Proceedings of the National Academy of Sciences of the United States of America·L J KornbergR L Juliano
Jan 1, 1991·Acta neurochirurgica·R SchoberW Wechsler
Aug 2, 1990·Nature·T A Springer
Jan 1, 1995·The Journal of Experimental Medicine·M R AldersonD H Lynch
Jul 28, 1995·The Journal of Biological Chemistry·F BerditchevskiM E Hemler
Jun 6, 1994·FEBS Letters·C MontecuccoG Schiavo
Jul 12, 1996·The Journal of Biological Chemistry·W Puzon-McLaughlinY Takada
Aug 1, 1996·The Journal of Cell Biology·S M FrischP Y Chan-Hui
Oct 1, 1996·Proceedings of the National Academy of Sciences of the United States of America·C WeberT A Springer
Feb 1, 1997·The Journal of Infectious Diseases·A Guichon, A Zychlinsky
Oct 23, 1997·Current Opinion in Cell Biology·F G Giancotti
Mar 4, 1998·Molecular Microbiology·J E KirbyR R Isberg
Apr 30, 1998·Current Opinion in Cell Biology·A HoweR L Juliano
Jul 24, 1998·Cellular and Molecular Life Sciences : CMLS·C M Longhurst, L K Jennings
Oct 29, 1998·Journal of Bacteriology·G R Cornelis
Aug 14, 1999·Science·F G Giancotti, E Ruoslahti

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Citations

Dec 10, 2013·Respiratory Research·Peng GaoPeter G Gibson
May 29, 2010·The Journal of Biological Chemistry·Nicole TegtmeyerSteffen Backert
Dec 18, 2003·The Journal of Biological Chemistry·Katherine ConantAvindra Nath
May 11, 2011·Reproductive Sciences·Huan YangWei Zhang
Jan 6, 2006·The Journal of Immunology : Official Journal of the American Association of Immunologists·Brianna N StillmanLinda G Baum
Mar 29, 2007·Cellular Microbiology·Andre Dubois, Thomas Borén
May 21, 2003·International Journal of Medical Microbiology : IJMM·Guntram A GrasslIngo B Autenrieth
May 21, 2003·International Journal of Medical Microbiology : IJMM·Thomas T MacDonaldCameron Simmons

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