PMID: 6983119Oct 1, 1982Paper

Induction of cell-mediated cytotoxic self responses by epidermal cells modified with a haptenic sulphydryl reagent

Scandinavian Journal of Immunology
H PehambergerS I Katz

Abstract

Murine epidermal cells (EC) act as stimulator cells in the generation of allogeneic cytotoxic T lymphocytes (CTL) in cell-mediated lympholysis (CML) and are suitable targets for allogeneic and hapten-self CTL. To analyse the role of EC in the generation of and recognition by anti-self CTL, syngeneic hapten-modified murine EC were used as in vivo and in vitro stimulating populations and as target cells in a hapten-self CML system. Epidermal cells were modified with the sulphydryl-reactive haptenic reagent N-iodoacetyl-N'-(5-sulphonic-1-naphthyl)ethylenediamine (I-AED). C3H.SW (H-2b) AED-self CTL responses were generated by stimulation with syngeneic AED-modified EC and were readily demonstrated when tested on syngeneic hapten-modified EC. These CML responses were hapten-specific and H-2 restricted. No substantial difference was detected in the ability of AED-modified EC and spleen cells (SC) to stimulate the generation of secondary AED-self CTL. Cold target inhibition experiments with hapten-modified EC and SC blockers did not reveal tissue-specific recognition of hapten-modified EC or SC targets by AED-self CTL. These findings demonstrate that hapten-modified EC, when used for priming in vivo and subsequently for in vitro sensi...Continue Reading

References

Apr 1, 1972·The Journal of Experimental Medicine·M ScheidL J Old
Jan 1, 1980·Immunological Reviews·G StinglS I Katz
Apr 1, 1981·The Journal of Investigative Dermatology·K TamakiS I Katz
Aug 1, 1981·The Journal of Investigative Dermatology·K TamakiS I Katz
Feb 1, 1980·The Journal of Experimental Medicine·W PtakR K Gershon
Apr 30, 1935·The Journal of Experimental Medicine·K Landsteiner, J Jacobs

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