Induction of chinook salmon growth hormone promoter activity by the adenosine 3',5'-monophosphate (cAMP)-dependent pathway involves two cAMP-response elements with the CGTCA motif and the pituitary-specific transcription factor Pit-1

Endocrinology
A O WongC L Hew

Abstract

In this study, the functional role of two cAMP-response elements (CRE) in the promoter of the chinook salmon GH gene and their interactions with the transcription factor Pit-1 in regulating GH gene expression were examined. A chimeric construct of the chloramphenicol acetyltransferase (CAT) reporter gene with the CRE-containing GH promoter (pGH.CAT) was transiently transfected into primary cultures of rainbow trout pituitary cells. The expression of CAT activity was stimulated by an adenylate cyclase activator forskolin as well as a membrane-permeant cAMP analog 8-bromo-cAMP. Furthermore, these stimulatory responses were inhibited by a protein kinase A inhibitor H89, suggesting that these CREs are functionally coupled to the adenylate cyclase-cAMP-protein kinase A cascade. This hypothesis is supported by parallel studies using GH4ZR7 cells, a rat pituitary cell line stably transfected with dopamine D2 receptors. In this cell line, D2 receptor activation is known to inhibit adenylate cyclase activity and cAMP synthesis. Stimulation with a nonselective dopamine agonist, apomorphine, or a D2-specific agonist, Ly171555, suppressed the expression of pGH.CAT in GH4ZR7 cells, and this inhibition was blocked by simultaneous treatment w...Continue Reading

Citations

Oct 19, 1999·The Journal of Clinical Investigation·L E CohenS Radovick
Jul 9, 2004·BMC Developmental Biology·Margarita V ChibalinaRobert H Insall
Jan 13, 2006·Comparative Biochemistry and Physiology. Part A, Molecular & Integrative Physiology·Anderson O L WongWendy K W Ko
Nov 18, 2005·General and Comparative Endocrinology·Shunsuke MoriyamaHiroshi Kawauchi
Aug 4, 1999·Molecular and Cellular Endocrinology·B SekkaliM Muller
Mar 1, 2008·Comparative Biochemistry and Physiology. Part D, Genomics & Proteomics·Ricardo AlmulyBruria Funkenstein
Jan 10, 2001·Canadian Journal of Physiology and Pharmacology·S HarveyK L Hull
Aug 19, 2000·Biochemistry and Cell Biology = Biochimie Et Biologie Cellulaire·A O WongJ P Chang
Jul 2, 2004·Experimental Biology and Medicine·Stephen C Y IpFrederick C Leung

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