Induction of choline acetyltransferase mRNA in human mononuclear leukocytes stimulated by phytohemagglutinin, a T-cell activator

Journal of Neuroimmunology
Takeshi FujiiKoichiro Kawashima

Abstract

The induction of mRNA for choline acetyltransferase (ChAT), which catalyzes acetylcholine (ACh) synthesis was investigated in human mononuclear leukocytes (MNL) stimulated by phytohemagglutinin (PHA), a T-cell activator, using the reverse transcription-polymerase chain reaction. Stimulation of MNL by PHA induced the expression of ChAT mRNA, and potentiated ACh synthesis. ChAT mRNA induction required more time than the induction of interleukin-2 mRNA. Expression of the gene encoding the vesicular ACh transporter, which mediates ACh transport in cholinergic neurons, was not observed in PHA-stimulated MNL, suggesting that the mechanisms controlling ACh release from T-lymphocytes differ from those in cholinergic neurons. These findings demonstrate that activation of T-lymphocytes up-regulates ACh synthesis in the blood, and suggest that ACh plays an important role as a neuroimmunomodulator besides its role as a neurotransmitter.

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Citations

May 26, 2005·Journal of Neuroimmunology·James C ZimringJudith A Kapp
Mar 12, 2003·Life Sciences·Koichiro Kawashima, Takeshi Fujii
Mar 12, 2003·Life Sciences·Hiroshi OgawaKoichiro Kawashima
Apr 13, 2000·Pharmacology & Therapeutics·K Kawashima, T Fujii
Sep 17, 2011·Science·Mauricio Rosas-BallinaKevin J Tracey
Apr 9, 2005·American Journal of Respiratory Cell and Molecular Biology·Katrin Susanne LipsHermann Koepsell
May 11, 2006·Respiratory Research·Reinoud GosensAndrew J Halayko
Mar 3, 2004·Nihon yakurigaku zasshi. Folia pharmacologica Japonica·Takeshi Fujii
Mar 13, 2001·Japanese Journal of Pharmacology·T Fujii, K Kawashima
Mar 13, 2001·Japanese Journal of Pharmacology·I WesslerC J Kirkpatrick
May 10, 2012·Life Sciences·Takeshi FujiiKoichiro Kawashima
Apr 25, 2015·International Immunopharmacology·Koichiro KawashimaKazuhide Horiguchi
Jan 17, 2012·Journal of Neuroimmunology·Takeshi FujiiKoichiro Kawashima
Mar 27, 2007·Life Sciences·Koichiro KawashimaHidemi Misawa
May 27, 2008·British Journal of Pharmacology·I Wessler, C J Kirkpatrick
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Jul 11, 2006·Journal of Neuroimmunology·Takeshi FujiiKoichiro Kawashima
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Nov 26, 2010·Neuropharmacology·Leonardo DionisioMaría Del Carmen Esandi
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Apr 11, 2017·Neuroscience and Biobehavioral Reviews·Bin HanJunwei Hao
Jul 22, 2014·Advances in Physiology Education·Eva Sundman, Peder S Olofsson
Sep 22, 2017·Frontiers in Immunology·Takeshi FujiiKoichiro Kawashima

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