Induction of chondrogenesis of human placenta-derived mesenchymal stem cells via heparin-grafted human fibroblast derived matrix

Biomaterials Research
Yong Kwan NohKwideok Park

Abstract

Formation of mature and functional articular cartilage is still challenging in cartilage tissue engineering. This study investigates the potential of using heparin-grafted decellularized extracellular matrix (ECM) as a novel growth factor delivery platform towards human placenta-derived mesenchymal stem cells (hPMSCs) chondrogenic differentiation. Human fibroblast-derived extracellular matrix (hFDM) is naturally obtained from in vitro-cultured human lung fibroblasts via a mild decellularization process. hFDM was then conjugated with heparin via N-(3-Dimethylaminopropyl)-N'-ethylcarbodiimide hydrochloride (EDC) chemistry and subject to transforming growth factor (TGF)-β1 immobilization. Once heparin grafted-hFDM (hFDM-hep) and hPMSCs were co-embedded into collagen gel, they were examined for in vitro and in vivo chondrogenesis of hPMSCs for 4 weeks. We identified heparin moieties on hFDM via toluidine blue O assay and Fourier transform infrared spectroscopy, respectively. We found out that collagen spheroids containing hFDM-hep and TGF-β1 exhibited a sustained release of growth factor for 28 days in vitro. Chondrogenesis of hPMSCs in vitro was supported by accumulated glycosaminoglycan (GAG) content and upregulated chondrogenic ...Continue Reading

References

Apr 2, 1999·Science·M F PittengerD R Marshak
Jul 22, 2006·Pharmaceutical Research·Hyun Jung ChungTae Gwan Park
Nov 21, 2008·Knee Surgery, Sports Traumatology, Arthroscopy : Official Journal of the ESSKA·Junji IwasaMitsuo Ochi
May 2, 2009·The Journal of Bone and Joint Surgery. British Volume·A GetgoodN Rushton
May 22, 2009·Nature Materials·Elsie S PlaceMolly M Stevens
Aug 24, 2010·Ageing Research Reviews·Yi-Ling SiWei-Dong Han
Nov 10, 2010·Expert Opinion on Biological Therapy·Takashi HoshibaGuoping Chen
Dec 3, 2010·Journal of Cell Science·Christian FrantzValerie M Weaver
Dec 15, 2010·Nature Nanotechnology·Tal DvirRobert Langer
Sep 21, 2012·World Journal of Stem Cells·Shalini VellasamyRajesh Ramasamy
Nov 20, 2012·Science·Daniel J HueyKyriacos A Athanasiou
Jul 11, 2013·Nature Reviews. Molecular Cell Biology·Fiona M Watt, Wilhelm T S Huck
Apr 22, 2014·Trends in Pharmacological Sciences·Gehua Zhen, Xu Cao
May 8, 2014·European Cells & Materials·M CucchiariniP Roughley
Sep 10, 2014·Journal of Controlled Release : Official Journal of the Controlled Release Society·Ping DuKwideok Park
Dec 23, 2014·Biomaterials Science·Lindsay E Fitzpatrick, Todd C McDevitt
Oct 14, 2016·Nature Reviews. Disease Primers·Johanne Martel-PelletierJean-Pierre Pelletier
Oct 27, 2017·Macromolecular Bioscience·Gabriella C J BrownTim B F Woodfield

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Citations

Dec 7, 2018·Journal of Materials Chemistry. B, Materials for Biology and Medicine·Chen-Yuan GaoXiao-Ping Yang

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Methods Mentioned

BETA
ELISA
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