PMID: 3748052Oct 1, 1986Paper

Induction of chromosome shattering by ultraviolet light and caffeine: the influence of different distributions of photolesions

Mutation Research
C Cremer, T Cremer

Abstract

Cells of synchronized and of asynchronously growing cultures of a V79 Chinese hamster line were microirradiated with a low power laser-UV-microbeam of wavelength 257 nm. Ultraviolet light was either focused onto a small part of the nucleus (mode I) or distributed over the whole nucleus (mode II). Following microirradiation, the cells were incubated for 7-20 h with caffeine (1-2 mM) until chromosome preparation was performed. After both modes of microirradiation, shattering of the entire chromosome complement (generalized chromosome shattering, GCS) was observed. It is suggested that the probability by which GCS is induced depends on the total number of DNA lesions rather than on their distribution in the chromatin. The results are consistent with the prediction of a "factor depletion model" which assumes that in a given cell, GCS takes place both in irradiated and non-irradiated chromosomes if the total number of daughter strand-repair sites surpasses a threshold value.

References

Dec 5, 1979·Journal of Molecular Biology·K Tatsumi, B S Strauss
Apr 1, 1985·Mutation Research·A González-FernándezJ F López-Sáez
May 1, 1970·Mutation Research·S C Barranco, R M Humphrey
Oct 1, 1972·Biophysical Journal·A R Lehmann
Dec 1, 1980·Proceedings of the National Academy of Sciences of the United States of America·R B Painter, B R Young
May 1, 1982·Proceedings of the National Academy of Sciences of the United States of America·C C Lau, A B Pardee

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Citations

Jun 19, 2009·European Biophysics Journal : EBJ·B HübnerT Cremer
Sep 7, 2001·Advances in Space Research : the Official Journal of the Committee on Space Research (COSPAR)·E NasonovaG Kraft
Feb 24, 2016·Microscopy and Microanalysis : the Official Journal of Microscopy Society of America, Microbeam Analysis Society, Microscopical Society of Canada·Michal FranekEva Bártová
Jan 31, 2012·Biochimica Et Biophysica Acta·Lei Shi, Philipp Oberdoerffer
Feb 18, 2003·Journal of Microscopy·J WalterS Tashiro
Sep 4, 2015·Journal of Biosciences·Guido A Drexler, Miguel J Ruiz-Gomez
Sep 30, 2016·The Journal of Histochemistry and Cytochemistry : Official Journal of the Histochemistry Society·Michal FranekStanislav Kozubek

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