PMID: 2815832Aug 1, 1989

Induction of cytochrome P-450 isozymes by chromanamine derivatives in rat liver

Xenobiotica; the Fate of Foreign Compounds in Biological Systems
K TsukidateY Kanakubo

Abstract

1. Pretreatment of rats with 6-(3-picolyl)amino-2,2,5,8-tetramethylchromane (PATC) for 7 days resulted in a significant increase in the activities of benzphetamine N-demethylase, p-nitroanisole O-demethylase and aniline hydroxylase in liver microsomes prepared 24 h after the last treatment. 2. Analysis by Western blot showed that PATC induces cytochrome P-450 b, P-450 c and P-450 d, which are the major forms of cytochrome P-450 in liver microsomes of rats when pretreated with phenobarbital and 3-methylcholanthrene. 3. Exposure of liver sections to the antibodies to cytochrome P-450 b and P-450 c resulted in intense immunostaining within the centrilobular regions, but produced staining of considerably weaker intensity in the perilobular region. Semiquantitative immunochemical analysis, by image analyser, of cytochrome P-450 b and P-450 c showed that centrilobular hepatocytes were stained more intensively than perilobular hepatocytes. 4. These results indicate that PATC induces cytochromes P-450 b and P-450 c, in the centrilobular hepatocytes to a greater degree than those in the perilobular hepatocytes. 5. Co-administration of PATC with pentobarbital caused a significant increase in pentobarbital sleeping time. Furthermore, PATC...Continue Reading

References

Mar 1, 1978·Chemico-biological Interactions·P E GoodingT F Slater
Jan 13, 1978·Biochemical and Biophysical Research Communications·M DickinsK J Netter
Dec 25, 1978·Life Sciences·J BaronF P Guengerich
May 1, 1973·Proceedings of the National Academy of Sciences of the United States of America·A P AlvaresA Kappas
Oct 1, 1972·Archives of Biochemistry and Biophysics·A Y LuA H Conney
Sep 16, 1974·Biochimica Et Biophysica Acta·A Y Lu, W Levin
Apr 1, 1981·The Journal of Histochemistry and Cytochemistry : Official Journal of the Histochemistry Society·S M HsuH Fanger

Citations

Aug 1, 1994·Xenobiotica; the Fate of Foreign Compounds in Biological Systems·T NaitohK Akasaka

Related Concepts

6-(3-picolyl)amino-2,2,5,8-tetramethylchromane
Benzopyrans
Epicholesterol
Chromans
Cytochrome P-450 Oxygenase
Pharmaceutical Preparations
Mixed Function Oxygenases
Immunocytochemistry
Alloenzymes
Liver

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