Induction of cytosolic Ca2+ elevation mediated by Mas-7 occurs through membrane pore formation.

The Journal of Biological Chemistry
B C SuhK T Kim

Abstract

Mas-7, a mastoparan derivative, induces elevation of intracellular free Ca2+ concentration ([Ca2+]i) along two independent pathways. The minor contribution occurs via phospholipase C activation and is negatively regulated by treatment with phorbol 12-myristate 13-acetate, a protein kinase C activator. The major contribution involves plasma membrane pores allowing not only Ca2+, Mn2+, and Na+ to enter but also the uptake of ethidium bromide (314 Da) and lucifer yellow (457 Da), but not fura-2 (831 Da), Evans blue (961 Da), and fluorescein-conjugate phalloidin (1,175 Da). Mas-7-induced current, as measured in planar lipid bilayers, reveals that Mas-7-induced pores have two slope conductances, 290 and 94 pS, and that the pores are nonselective for cations. The results also indicate that Mas-7 can produce pores by direct interaction with the plasma membrane without the involvement of membrane proteins and cytosolic factors. Besides in human neuroblastoma cells, similar Mas-7 effects were also observed in other cell lines such as HL-60, 1321N1 human astrocytoma, and bovine chromaffin cells. The data suggest that the Mas-7-induced [Ca2+]i elevation is the combined result of Ca2+ release from stores via phosphoinositide turnover and p...Continue Reading

References

Aug 3, 1992·European Journal of Pharmacology·R A CrucianiE F Stanley
Sep 1, 1991·The Biochemical Journal·B M DenkerE J Neer
Jan 1, 1991·Journal of Biochemistry·U TomitaT Katada
Jun 14, 1991·Biochemical and Biophysical Research Communications·A TanimuraY Tojyo
Jul 31, 1990·Biochemical and Biophysical Research Communications·Y OzakiS Kume
Aug 24, 1990·Biochimica Et Biophysica Acta·T KatsuY Fujita
Jan 29, 1990·FEBS Letters·T NagasawaT Abe
Feb 15, 1989·Biochemical and Biophysical Research Communications·N YokokawaT Yamada
Jan 1, 1989·Proceedings of the National Academy of Sciences of the United States of America·M T TostesonD C Tosteson
May 19, 1989·Biochimica Et Biophysica Acta·K MatsuzakiK Miyajima
Mar 17, 1972·Biochimica Et Biophysica Acta·L G Gordon, D A Haydon
Sep 1, 1980·Journal of Neurochemistry·D L KilpatrickN Kirshner
Jul 18, 1983·Biochemical and Biophysical Research Communications·D A Malencik, S R Anderson
Apr 17, 1995·Biochemical and Biophysical Research Communications·J F Klinker, R Seifert
Mar 3, 1995·The Journal of Biological Chemistry·D R PfeifferW L Erdahl
Nov 15, 1993·European Journal of Pharmacology·D L SongC H Chang
Mar 1, 1994·Trends in Pharmacological Sciences·C FasolatoT Pozzan
Nov 15, 1993·Biochemical and Biophysical Research Communications·M DanilenkoY Sharoni
Jan 28, 1993·Nature·M J Berridge
Jan 4, 1996·Biochimica Et Biophysica Acta·C C ChuangS H Wu

❮ Previous
Next ❯

Citations

Jan 16, 2003·Cell Calcium·Maria Giovanna EliaSanto Marsigliante
Mar 2, 2005·Photochemical & Photobiological Sciences : Official Journal of the European Photochemistry Association and the European Society for Photobiology·Alexandre Fürstenberg, Eric Vauthey
Feb 15, 2002·The Plant Journal : for Cell and Molecular Biology·Marc J Curtis, Thomas J Wolpert
Feb 18, 2016·Neural Plasticity·Valerie T RamírezNibaldo C Inestrosa
Apr 28, 2004·Toxicon : Official Journal of the International Society on Toxinology·Dan GincelFrederic Libersat
Sep 3, 2005·Analytical Biochemistry·Külliki SaarUlo Langel
Mar 9, 2004·Mechanisms of Development·Ekaterina Voronina, Gary M Wessel
Dec 3, 2014·PloS One·Francesca LangoneGianni Cesareni
May 22, 2001·The Journal of Immunology : Official Journal of the American Association of Immunologists·B C SuhK T Kim
Jul 24, 2001·The Journal of Immunology : Official Journal of the American Association of Immunologists·B C SuhK T Kim

❮ Previous
Next ❯

Related Concepts

Related Feeds

ASBMB Publications

The American Society for Biochemistry and Molecular Biology (ASBMB) includes the Journal of Biological Chemistry, Molecular & Cellular Proteomics, and the Journal of Lipid Research. Discover the latest research from ASBMB here.

Astrocytes

Astrocytes are glial cells that support the blood-brain barrier, facilitate neurotransmission, provide nutrients to neurons, and help repair damaged nervous tissues. Here is the latest research.

Calcium & Bioenergetics

Bioenergetic processes, including cellular respiration and photosynthesis, concern the transformation of energy by cells. Here is the latest research on the role of calcium in bioenergetics.