Induction of dark keratinocytes by 12-O-tetradecanoylphorbol-13-acetate and mezerein as an indicator of tumor-promoting efficiency

Carcinogenesis
A J Klein-SzantoT J Slaga

Abstract

12-O-Tetradecanoylphorbol-13-acetate (TPA) and mezerein (MZ) are diterpene esters of similar structure and approximately equipotent on a molar basis as far as their hyperplasiogenic, inflammatory, and induction of ornithine decarboxylase activity effects in mouse skin are concerned. On the other hand, TPA is much more effective than MZ as a tumor promoter. The percentage of dark basal keratinocytes was determined in the interfollicular epidermis (IFE) of mice topically treated with 1, 2, or 4 microgram of either TPA or MZ in a single application and studied at 12, 24, 48, 96, and 144 h thereafter. The results showed that TPA induced 2 to 3 times more dark cells than MZ in the IFE as well as in the infundibular portion of the hair follicle. The latter epithelium presented a larger number of dark keratinocytes than the IFE in all experimental and control situations, and the differences between the effects of TPA and MZ were even greater in the infundibular epidermis than in the IFE. TPA induced an increase of 5 to 11 times over the control number of dark cells (approximately 2% in IFE), reaching maximum values of 21% in the basal layer 24 h after topical application of 4 microgram of TPA. MZ only produced a 3- to 6-fold increment...Continue Reading

Citations

Jan 1, 1995·Teratogenesis, Carcinogenesis, and Mutagenesis·M BlessingB L Hogan
Jan 1, 1983·Archives of Dermatological Research·G Kolde, F Vakilzadeh
Jan 1, 1992·Pharmacology & Therapeutics·J DiGiovanni
Apr 1, 1983·Environmental Health Perspectives·T J Slaga
Nov 1, 1991·In Vitro Cellular & Developmental Biology : Journal of the Tissue Culture Association·R J MorrisT J Slaga
May 1, 1981·Critical Reviews in Toxicology·T S Argyris
Jan 1, 1985·Critical Reviews in Toxicology·T S Argyris
Jan 1, 1984·Cancer Investigation·O H Iversen, E G Astrup
Mar 1, 1982·Stain Technology·D F ParsonsM Marko
Apr 1, 1990·Journal of Cutaneous Pathology·D FeldmanS S Shapiro
Oct 3, 2009·Food and Chemical Toxicology : an International Journal Published for the British Industrial Biological Research Association·Kausar M Ansari, Mukul Das
Jul 1, 1985·Cancer Letters·S M FischerT J Slaga
Jun 1, 1982·Journal of Oral Pathology·A J Klein-SzantoM Albrecht
Jan 1, 1982·Journal of Cellular Biochemistry·T J SlagaJ Reiners
Apr 1, 1981·Journal of Cutaneous Pathology·B M de ReyM E Itoiz
Feb 1, 1982·Journal of Cutaneous Pathology·T S Argyris
May 29, 1986·Biochemical and Biophysical Research Communications·T KurokiY Murakami
Jan 1, 1981·Journal of Supramolecular Structure and Cellular Biochemistry·S H YuspaU Lichti
Jan 1, 1994·Journal of Toxicology. Clinical Toxicology·K R Hardy, S R Thom

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.