Induction of defence gene expression by oligogalacturonic acid requires increases in both cytosolic calcium and hydrogen peroxide in Arabidopsis thaliana

Cell Research
Xiangyang HuZhangcheng Tang

Abstract

Responses to oligogalacturonic acid (OGA) were determined in transgenic Arabidopsis thaliana seedlings expressing the calcium reporter protein aequorin. OGA stimulated a rapid, substantial and transient increase in the concentration of cytosolic calcium ([Ca2+]cyt) that peaked after ca. 15 s. This increase was dose-dependent, saturating at ca. 50 ug Gal equiv/ml of OGA. OGA also stimulated a rapid generation of H2O2. A small, rapid increase in H2O2 content was followed by a much larger oxidative burst, with H2O2 content peaking after ca. 60 min and declining thereafter. Induction of the oxidative burst by OGA was also dose-dependent, with a maximum response again being achieved at ca. 50 ug Gal equiv/mL. Inhibitors of calcium fluxes inhibited both increases in [Ca2+]cyt and [H2O2], whereas inhibitors of NADPH oxidase blocked only the oxidative burst. OGA increased strongly the expression of the defence-related genes CHS, GST, PAL and PR-1. This induction was suppressed by inhibitors of calcium flux or NADPH oxidase, indicating that increases in both cytosolic calcium and H2O2 are required for OGA-induced gene expression.

References

Aug 1, 1973·Analytical Biochemistry·N Blumenkrantz, G Asboe-Hansen
Mar 15, 1997·The Biochemical Journal·P Wojtaszek
Oct 10, 1997·The Journal of Hospital Infection·C M ParryC A Hart
Mar 6, 1999·Current Opinion in Plant Biology·A J Trewavas, R Malhó
Mar 9, 1999·The Plant Journal : for Cell and Molecular Biology·H KnightM R Knight
Aug 25, 1999·Current Opinion in Plant Biology·G P Bolwell
Feb 13, 2001·Plant Science : an International Journal of Experimental Plant Biology·A S.N. Reddy
May 30, 2001·Trends in Plant Science·H Knight, M R Knight
Sep 13, 2001·Plant Physiology·R DesikanS J Neill
May 9, 1995·Proceedings of the National Academy of Sciences of the United States of America·S H DoaresC A Ryan
Jan 5, 2002·Proceedings of the National Academy of Sciences of the United States of America·Miguel Angel TorresJonathan D G Jones
May 9, 2002·Journal of Experimental Botany·Steven J NeillJohn T Hancock
Jun 1, 1997·Annual Review of Plant Physiology and Plant Molecular Biology·Chris Lamb, Richard A. Dixon
Mar 1, 2001·The New Phytologist·M Tang, C J Smith

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Citations

Jan 24, 2006·Plant Physiology·Charlotte J SongStanley J Roux
Aug 24, 2007·Cellular Microbiology·Wei Ma, Gerald A Berkowitz
Jul 27, 2006·The New Phytologist·David LecourieuxAlain Pugin
Aug 21, 2013·The Plant Journal : for Cell and Molecular Biology·Hamid ManzoorAngela Garcia-Brugger
Aug 4, 2009·Plant Signaling & Behavior·Patrice MeimounFrançois Bouteau
Mar 17, 2021·Nature Plants·Marta BjornsonCyril Zipfel
Oct 14, 2021·International Journal of Molecular Sciences·Maxim MudrilovVladimir Vodeneev
Nov 5, 2021·Frontiers in Plant Science·Shivani KansalSaurabh Raghuvanshi

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