Induction of differentiation in HL60 leukaemic cells: a cell cycle dependent all-or-none event

Leukemia Research
A W Boyd, D Metcalf

Abstract

The human leukaemia cell line (HL60) shows a limited capacity to differentiate spontaneously, but this property can be greatly enhanced by chemical inducers. Sodium butyrate induced differentiation in virtually 100% of HL60 cells over a four-day interval to cells with multiple phenotypic markers of monocytes. Clonogenic analysis in agar demonstrated that differentiated cells (either spontaneous or induced) irreversibly lost clonogenic potential. This appeared to be an all-or-none process with unaffected cells exhibiting unaltered clonogeneity. A study of the kinetics of colony formation showed that most, if not all, cells completed one division in the presence of butyrate and sometimes several divisions before loss of proliferative potential. Despite the uniform spectrum of cell cycle states present in HL60 cultures when butyrate was added, all differentiated cells were shown to be arrested in G1. Evidence was obtained suggesting that the 'switch' into the differentiation pathway occurred during a restricted stage of the cell cycle, either late in the cycle (G2-M) or early in G1.

References

Jun 1, 1979·Proceedings of the National Academy of Sciences of the United States of America·G RoveraC Damsky
Jan 1, 1976·Journal of Immunological Methods·J W Goding
Apr 1, 1974·Proceedings of the National Academy of Sciences of the United States of America·A B Pardee
Dec 1, 1981·Cell·S C Elgin
Nov 1, 1980·Proceedings of the National Academy of Sciences of the United States of America·Z DarzynkiewiczM R Melamed
Apr 7, 1983·Journal of Theoretical Biology·A W Boyd
Jul 1, 1981·Proceedings of the National Academy of Sciences of the United States of America·T Naveh-Many, H Cedar
Jul 1, 1982·Cell and Tissue Kinetics·P FoaE E Polli
Oct 1, 1982·Journal of Cellular Physiology·E FibachE A Rachmilewitz
Nov 1, 1980·The Journal of Experimental Medicine·W JuddJ L Strominger
May 1, 1981·Cell·M Groudine, H Weintraub
Aug 28, 1980·Nature·M B OmaryJ Minowada

❮ Previous
Next ❯

Citations

Jan 1, 1996·Leukemia Research·G A HairF R Rickles
Mar 1, 1992·Molecular and Cellular Endocrinology·A C Maiyar, A W Norman
Jul 24, 2002·Experimental Hematology·Claudia C Gregorio-KingMark A Kirkland
Aug 25, 2001·Cancer Letters·O WittA Pekrun
Aug 1, 1989·Proceedings of the National Academy of Sciences of the United States of America·C NerviA M Jetten
Jun 2, 2012·Evidence-based Complementary and Alternative Medicine : ECAM·Ramani SoundararajanAparna Dixit
Mar 29, 2014·PloS One·Agnese RavettoCarlijn V C Bouten
Jun 12, 2013·PLoS Genetics·Marit AckermannAndreas Beyer
Jan 1, 1985·Leukemia Research·R E GallagherE C Muly
Jan 1, 1986·Differentiation; Research in Biological Diversity·A LadouxB Geny
Apr 26, 2003·Journal of Leukocyte Biology·Mohammad J AbedinMitsuomi Hirashima
Aug 1, 1988·Australian and New Zealand Journal of Medicine·E H Januszewicz, F C Firkin
Mar 12, 1990·Philosophical Transactions of the Royal Society of London. Series B, Biological Sciences·D Metcalf
Jan 12, 2005·Clinical and Diagnostic Laboratory Immunology·R A FleckM H Nahm
Jan 15, 1987·International Journal of Cancer. Journal International Du Cancer·C G BegleyN A Nicola
Sep 1, 1988·Journal of Clinical Pharmacology·E L Schwartz, P H Wiernik

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.