PMID: 12770180May 29, 2003Paper

Induction of digestive alpha-amylases in larvae of Zabrotes subfasciatus (Coleoptera: Bruchidae) in response to ingestion of common bean alpha-amylase inhibitor 1

Journal of Insect Physiology
C P SilvaJ S. Almeida

Abstract

Zabrotes subfasciatus larvae possess three alpha-amylase isoforms determined by in gel assays following SDS-PAGE. Two minor isoforms present lower electrophoretic mobility than the major form. When developed inside Vigna unguiculata (cowpea) seeds, fourth instar larvae have minor quantities of the slow-migrating isoforms, but when reared on seeds of Phaseolus vulgaris (common bean), the two slow-migrating forms are expressed in higher amounts, whilst the quantity of the major constitutive form is independent of the host bean. Larvae at the beginning of the fourth instar were fed on flour or cotyledons of cowpea and common bean and it was observed that the larvae fed on the common bean expressed the two slow-migrating forms in higher amounts when compared to the control larvae fed on cowpea. In order to investigate the possible correlation between the induction of alpha-amylases and the ingestion of the common bean alpha-amylase inhibitor 1 (alphaAI-1), this inhibitor was incorporated into artificial diet. It was observed that larvae fed on diet containing chronic doses of alphaAI-1 during their development, produced the two slow-migrating forms in higher amounts than control larvae, however, fourth-instar larvae fed on the same...Continue Reading

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Citations

May 13, 2014·Comparative Biochemistry and Physiology. Part B, Biochemistry & Molecular Biology·Daniel AlexandreCarlos P Silva
Apr 9, 2008·Comparative Biochemistry and Physiology. Part B, Biochemistry & Molecular Biology·Francisco J Cinco-MoroyoquiFrancisco J Wong-Corral
Dec 23, 2006·TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik·Keito NishizawaMasao Ishimoto
Oct 19, 2007·Comparative Biochemistry and Physiology. Part B, Biochemistry & Molecular Biology·Biljana DojnovZoran Vujcić
Apr 8, 2020·Toxicological Research·Fatma Zohra AmeurDjamel Saidi
Feb 21, 2006·Comparative Biochemistry and Physiology. Part A, Molecular & Integrative Physiology·Maria Lígia Rodrigues MacedoLuana Cassandra Breitenbach Barroso Coelho
Oct 5, 2013·Journal of Proteome Research·Vishal V DawkarAshok P Giri

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