Induction of DNA methylation by artificial piRNA production in male germ cells

Current Biology : CB
Daisuke ItouToru Nakano

Abstract

Global DNA demethylation and subsequent de novo DNA methylation take place in mammalian male embryonic germ cells [1-3]. P-element-induced wimpy testis (PIWI)-interacting RNAs (piRNAs), which are germline-specific small RNAs, have been postulated to be critically important for de novo DNA methylation of retrotransposon genes, and many proteins, including PIWI family proteins, play pivotal roles in this process [4-6]. In the embryonic mouse testis, two mouse PIWI proteins, mouse PIWI-like (MILI) and mouse PIWI2 (MIWI2), are involved in the biogenesis of piRNAs through the so-called ping-pong amplification cycle [7-10], and long single-stranded RNAs transcribed from the gene regions of piRNA clusters have been proposed to be the initial material [11-16]. However, it remains unclear whether transcription from the piRNA clusters is required for the biogenesis of piRNAs. To answer this question, we developed a novel artificial piRNA production system by simple expression of sense and antisense EGFP mRNAs in embryonic male germ cells in the piRNA biogenesis phase. EGFP expression was silenced by piRNA-dependent DNA methylation, indicating that concomitant expression of sense and antisense RNA transcripts is necessary and sufficient f...Continue Reading

References

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Citations

Jan 14, 2016·Critical Reviews in Clinical Laboratory Sciences·Laila C SchenkelBekim Sadikovic
Nov 20, 2015·Nucleic Acids Research·David Rosenkranz
Jan 16, 2016·Molecular Cancer·Kevin W NgVictor D Martinez
Mar 2, 2016·BioEssays : News and Reviews in Molecular, Cellular and Developmental Biology·Melvin M BonillaDavid W Zeh
Apr 2, 2015·Current Biology : CB·Takamasa Hirano, Haruhiko Siomi
Aug 22, 2015·Current Biology : CB·Satomi Kuramochi-Miyagawa, Toru Nakano
Oct 30, 2016·Proceedings of the National Academy of Sciences of the United States of America·Sayan NandiEric R Kandel
Aug 23, 2016·World Journal of Gastroenterology : WJG·Meng Zhang, Xiang Du
Jul 18, 2017·Diabetologia·Imène Sarah HenaouiRomano Regazzi
Nov 12, 2017·Nature Communications·Christina ErnstClaudia Kutter
Dec 14, 2018·Genes·Marthe LaisnéPierre-Antoine Defossez
May 5, 2018·International Journal of Molecular Sciences·Ariel Ernesto Cariaga-MartínezRaúl Alelú-Paz
Oct 20, 2020·Biochimica Et Biophysica Acta. Reviews on Cancer·Jie WangJinming Li
Feb 13, 2021·Biochemical Pharmacology·B BaptistaF Sousa

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