PMID: 2120408Aug 1, 1990Paper

Induction of DNA strand breaks and DNA-protein cross-links in normal human skin fibroblasts following exposure to 254 nm UV radiation

Journal of Photochemistry and Photobiology. B, Biology
L W Lai, B S Rosenstein

Abstract

Levels of DNA strand breaks and DNA-protein cross-links (DPCs) were measured using the alkaline elution assay in normal human skin fibroblasts irradiated with 0-200 J m-2 of 254 nm UV radiation and incubated for 0-24 h. On incubation, the yields of both single-strand breaks (SSBs) and DPCs increased with similar kinetics and remained elevated. In addition, when SSBs were measured under conditions in which DPCs were not eliminated by treatment with proteinase K, a measurable yield of SSBs could not be detected. Hence, the SSBs that form in the UV-irradiated cells following incubation appear to be associated with the DPCs.

References

Oct 19, 1976·Biochemistry·K W KohnC A Friedman
Nov 30, 1987·Biochemical and Biophysical Research Communications·R MohamedM F Lavin
May 11, 1988·Nucleic Acids Research·S P SinghM F Lavin
Jan 1, 1985·Annual Review of Biochemistry·J C Wang
Nov 1, 1987·Proceedings of the National Academy of Sciences of the United States of America·H H EvansM F Horng
Jul 1, 1987·Photochemistry and Photobiology·L W LaiB S Rosenstein
Apr 1, 1983·Proceedings of the National Academy of Sciences of the United States of America·A M Pedrini, G Ciarrocchi
Mar 1, 1984·Proceedings of the National Academy of Sciences of the United States of America·E M NelsonL F Liu
Dec 1, 1984·International Journal of Radiation Biology and Related Studies in Physics, Chemistry, and Medicine·S M ChiuN L Oleinick

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Citations

Sep 15, 1992·Journal of Photochemistry and Photobiology. B, Biology·J CadetL Voituriez
Apr 1, 1992·The Journal of Investigative Dermatology·B S RosensteinG B Zamansky

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