PMID: 9524943Apr 3, 1998Paper

Induction of dog sperm capacitation by oviductal fluid

The Journal of Veterinary Medical Science
Eiichi KawakamiToshihiko Tsutsui

Abstract

Four estrous beagles were inseminated with 1 x 10(8) sperm into both the right and left uterine horns, and the uterine horn and oviduct on one side were removed under anesthesia after 7 hr and 24 hr, respectively. The lumen of the uterine horns and oviducts was flushed with canine capacitation medium (CCM), and movement of the sperm in CCM was assessed by phase-contrast microscopy. In a second experiment, ejaculated sperm obtained from 5 normal beagles was incubated in CCM supplemented with oviductal flush fluid (OF-CCM) at 38 degrees C with 5% CO2 in air. Motility of sperm, and percentages of hyperactivated sperm (%HA) and acrosome-reacted sperm (%AR) among freely swimming (FS) sperm were investigated until 24 hr after the start of incubation. After 7 hr of incubation the sperm was coincubated with canine oocytes in OF-CCM for 2 hr, and the number of zona pellucida-binding (zona-binding) sperm was then counted. The %HA among the sperm in the oviductal flush fluid both 7 hr (mean +/- S.E.; 15.0 +/- 2.4%) and 24 hr (77.5 +/- 5.2%) after intrauterine insemination were significantly higher than in the uterine flush fluid (P < 0.05, 0.01, respectively). The motility and %HA among FS-sperm in OF-CCM were higher than in the control m...Continue Reading

References

May 1, 1976·The Journal of Experimental Zoology·C A Mahi, R Yanagimachi
Jun 1, 1991·The Journal of Veterinary Medical Science·E KawakamiA Ogasa
Dec 1, 1991·International Journal of Andrology·J F GuerinY Menezo
Mar 1, 1991·Journal of Reproduction and Fertility·T T Smith, R Yanagimachi
Apr 1, 1989·Nihon juigaku zasshi. The Japanese journal of veterinary science·T TsutsuiA Ogasa
May 1, 1989·Biology of Reproduction·J J ParrishN L First
Feb 1, 1987·Biology of Reproduction·S S Suarez
Jan 1, 1986·The Journal of Experimental Zoology·S Meizel, K O Turner
Feb 1, 1973·Biology of Reproduction·R D PhemisterM L Hopwood
Oct 1, 1971·Biology of Reproduction·P A Holst, R D Phemister
Feb 1, 1967·Journal of Reproduction and Fertility·R L DoakH E Dale
Feb 1, 1981·The Journal of Experimental Zoology·P Talbot, R S Chacon
May 1, 1980·Biology of Reproduction·D F Katz, R Vanagimachi
Jan 1, 1994·Molecular Reproduction and Development·A IjazM A Sirard
Apr 1, 1993·Biology of Reproduction·E KawakamiJ W Overstreet

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Citations

Sep 6, 2014·Reproduction : the Official Journal of the Society for the Study of Fertility·Sergio GhersevichCarlos Zumoffen
Mar 2, 2013·PloS One·Sarah L Freeman, Gary C W England
Apr 23, 2005·The Journal of Reproduction and Development·Ann-Sofi BergqvistHeriberto Rodríguez-Martínez
Mar 17, 2006·Theriogenology·G C W EnglandA A Pacey
Aug 16, 2005·Animal Reproduction Science·Ann-Sofi Bergqvist, Heriberto Rodríguez-Martínez
Jun 10, 2004·The Journal of Veterinary Medical Science·Eiichi KawakamiToshihiko Tsutsui
Aug 20, 2002·The Journal of Veterinary Medical Science·Eiichi KawakamiToshihiko Tsutsui
Jul 11, 2006·Zygote : the Biology of Gametes and Early Embryos·Ann-Sofi BergqvistHeriberto Rodríguez-Martínez
Jul 28, 2020·Molecular and Cellular Endocrinology·Marie Saint-DizierXavier Druart

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