Induction of dopaminergic neurotransmitter phenotype in rat embryonic cerebrocortex by the synergistic action of neurotrophins and dopamine

The European Journal of Neuroscience
J ZhouG M Stern

Abstract

Neurotrophins, including nerve growth factor, brain-derived neurotrophic factor (BDNF), neurotrophin-3 (NT-3) and neurotrophin-4/5 (NT-4/5), have been shown to enhance survival and differentiation of a variety of central neuronal populations, such as those with the dopaminergic, cholinergic, GABAergic phenotype during development. In this paper we present evidence that BDNF, NT-3 and NT-4/5 acting synergistically with dopamine (DA) can artificially induce the full dopaminergic phenotype in rat fetal cerebral cortex which normally has very few dopaminergic neurons in adulthood. Thus, BDNF/DA, NT-3/DA, NT-4/DA elicited a great increase in the number of tyrosine hydroxylase (TH)-immunoreactive cells, which was up to 5-7% of total neuronal population in cultures of fetal rat cortical cells. This stimulatory effect was not dependent on glial proliferation, or on addition of serum to the culture. Pharmacological studies showed that dopamine receptors D1 and D2 were involved in this effect. The TH+ cortical cells possessed other biochemical phenotypic features of dopaminergic neurons. Thus, high-affinity DA uptake was elevated in cortical cultures treated with neurotrophin/DA. Also DA and 3,4-dihydroxyphenlacetic acid production was d...Continue Reading

References

Jan 1, 1978·Annual Review of Neuroscience·P H Patterson
Oct 1, 1979·Proceedings of the National Academy of Sciences of the United States of America·A ProchiantzJ Glowinski
Aug 1, 1990·Trends in Neurosciences·S C Landis
Apr 24, 1991·Brain Research. Developmental Brain Research·K J MackR D Todd
Jun 1, 1990·Trends in Pharmacological Sciences·P H AndersenM G Caron
Dec 1, 1989·Journal of Neurochemistry·R Spector
Jan 1, 1995·Annual Review of Neuroscience·F H GageL J Fisher
Nov 1, 1994·Journal of Neurobiology·M Barbacid
Mar 1, 1993·Proceedings of the National Academy of Sciences of the United States of America·C Lois, A Alvarez-Buylla
Feb 15, 1996·Journal of Neuroscience Research·B S ReinosoP Levitt
Jan 1, 1992·Reviews in the Neurosciences·P A LapchakF Hefti

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Citations

Dec 13, 2000·Brain Research. Brain Research Reviews·D O Frost, J L Cadet
Sep 15, 2001·Brain Research. Developmental Brain Research·N D StullL Iacovitti
May 20, 1997·Brain Research. Developmental Brain Research·J ZhouG M Stern
May 13, 1999·Brain Research. Developmental Brain Research·F B Pliego RiveroH F Bradford
Oct 16, 1999·Brain Research. Developmental Brain Research·J C CoprayJ Lipski
Sep 18, 1998·Neuroscience Letters·J ZhouE R Jauniaux
Mar 30, 2007·Neuropsychopharmacology : Official Publication of the American College of Neuropsychopharmacology·Regina HünnerkopfKlaus Peter Lesch
Nov 28, 2002·The European Journal of Neuroscience·Hong GuoJiawei Zhou
Dec 14, 1999·The European Journal of Neuroscience·S R SinclairS B Dunnett
Jul 24, 2010·Psychoneuroendocrinology·J P CurleyF A Champagne
Oct 7, 2004·Brain Research. Developmental Brain Research·Samina S RiazHenry F Bradford
Mar 26, 2003·Brain Research·Julian GoggiHenry F Bradford
Oct 26, 2005·Molecular and Cellular Neurosciences·Gonçalo Castelo-BrancoErnest Arenas

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