PMID: 11326421May 1, 2001Paper

Induction of drug metabolism-related enzymes by methylcholanthrene and phenobarbital in transgenic mice carrying human prototype c-Ha-ras gene and their wild type littermates

Experimental Animals
Y OhnishiY Ueyama

Abstract

Transgenic mice hemizygously carrying human c-Ha-ras proto-oncogene, Tg-rasH2 show very sensitive and facilitated carcinogenicity to various carcinogens. In this study, activities of certain enzymes related to drug metabolism and energy metabolism were measured in microsome and cytosol fractions of livers of Tg-rasH2 mice and their wild type littermates with both sexes treated with 3-methylcholanthrene (MC) and phenobarbital (PB). Aminopyrine N-demethylase activities increased significantly in livers of all mice treated with PB. MC and PB treatments induced significant increases in activities of UDP-glucuronosyltransferase and S-adenosyl homocysteinase compared to those in the non-treated groups in microsome fractions from all mice. In cytosol fractions of livers of all mice, glutathione S-transferase activity was significantly induced in the PB treated groups. There were no significant differences in activities of lactate dehydrogenase, glucose 6-phosphate dehydrogenase, pyruvate kinase and glucose 6-phosphatase related to energy metabolism in livers and kidneys among all mice. Tg-rasH2 mice showed stable activities of enzymes related to drug detoxication and energy metabolism similar to those of non-transgenic mice. These res...Continue Reading

References

Sep 1, 1994·International Journal of Cancer. Journal International Du Cancer·N ShinoharaN Kuzumaki
Jan 1, 1997·Research in Veterinary Science·T AraiM Washizu
Nov 14, 1997·Journal of Veterinary Pharmacology and Therapeutics·V RátzG Semjén

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Citations

Jul 13, 2007·Xenobiotica; the Fate of Foreign Compounds in Biological Systems·S P RiveraO Hankinson
Dec 25, 2002·The Journal of Veterinary Medical Science·Toshiro AraiYasuyuki Ohnishi

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