Induction of efficient energy dissipation in the isolated light-harvesting complex of Photosystem II in the absence of protein aggregation.

The Journal of Biological Chemistry
Cristian IlioaiaAlexander V Ruban

Abstract

Under excess illumination, the Photosystem II light-harvesting antenna of higher plants has the ability to switch into an efficient photoprotective mode, allowing safe dissipation of excitation energy into heat. In this study, we show induction of the energy dissipation state, monitored by chlorophyll fluorescence quenching, in the isolated major light-harvesting complex (LHCII) incorporated into a solid gel system. Removal of detergent caused strong fluorescence quenching, which was totally reversible. Singlet-singlet annihilation and gel electrophoresis experiments suggested that the quenched complexes were in the trimeric not aggregated state. Both the formation and recovery of this quenching state were inhibited by a cross-linker, implying involvement of conformational changes. Absorption and CD measurements performed on the samples in the quenched state revealed specific alterations in the spectral bands assigned to the red forms of chlorophyll a, neoxanthin, and lutein 1 molecules. The majority of these alterations were similar to those observed during LHCII aggregation. This suggests that not the aggregation process as such but rather an intrinsic conformational transition in the complex is responsible for establishment ...Continue Reading

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Citations

Jul 23, 2013·Physical Chemistry Chemical Physics : PCCP·C D P DuffyA V Ruban
Jun 19, 2013·Physical Chemistry Chemical Physics : PCCP·Erica BelgioAlexander V Ruban
Nov 3, 2010·The Journal of Biological Chemistry·Cristian IlioaiaAlexander V Ruban
Nov 14, 2012·Philosophical Transactions of the Royal Society of London. Series B, Biological Sciences·Peter Horton
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Oct 23, 2014·Journal of Experimental Botany·Alexander V Ruban
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Jul 6, 2014·Langmuir : the ACS Journal of Surfaces and Colloids·Cvetelin VasilevC Neil Hunter
Sep 26, 2015·Journal of Photochemistry and Photobiology. B, Biology·Christopher D P Duffy, Alexander V Ruban
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Mar 31, 2009·Biochimica Et Biophysica Acta·Tiago Barros, Werner Kühlbrandt
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Jun 11, 2009·Communicative & Integrative Biology·Alexander V Ruban
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May 26, 2018·FEBS Letters·Alexander V Ruban

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