PMID: 9186586Apr 15, 1997Paper

Induction of endothelial platelet-derived growth factor-B-chain and intercellular adhesion molecule-1 by lysophosphatidylcholine

Annals of the New York Academy of Sciences
T KitaM Yokode

Abstract

Lysophosphatidylcholine (lyso-PC) is a major phospholipid component of atherogenic lipoproteins. Lyso-PC has been shown to differentially upregulate the adhesion molecules, such as VCAM-1 and ICAM-1, as well as smooth muscle growth factors, such as PDGF-A, B chains and HB-EGF gene expression in various cultured endothelial cells. In this paper, we demonstrate increased expression of cell- and matrix-associated forms of PDGF-B protein elicited by lyso-PC and further characterized potential signal transduction mechanisms responsible for lyso-PC-induced human umbilical vein endothelial cell. Cycloheximide inhibited PDGF-B but not ICAM-1 mRNA induction by lyso-PC, suggesting the dependence on de novo protein synthesis for PDGF-B, but not ICAM-1. A protein kinase C (PKC) inhibitor did not block lyso-PC-induced increases in PDGF-B or ICAM-1 mRNA. The elevated level of cAMP blocked both PDGF-B and ICAM-1 upregulation by lyso-PC. However cAMP-elevating agents did not suppress ICAM-1 upregulation by PMA. Taken together, PDGF-B and ICAM-1 gene induction by lyso-PC may involve different signaling mechanisms; however, both appear to be independent of PMA-regulatable PKC activation but are suppressed by increased levels of intracellular cAMP.

References

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Citations

Jul 30, 2002·Journal of Biomedical Science·Tung-Yi HuangChauying J Jen
Nov 5, 2003·Journal of Molecular and Cellular Cardiology·Gurunathan MurugesanKandice Kottke-Marchant
Dec 18, 2002·Annals of the New York Academy of Sciences·Hans W M NiessenC Erik Hack
Aug 8, 2012·Biochimica Et Biophysica Acta·Ioanna SevastouVassilis Aidinis
Nov 10, 2015·APMIS : Acta Pathologica, Microbiologica, Et Immunologica Scandinavica·Wahid Ali Khan, Javed Anwer Qureshi
Jun 18, 2002·American Journal of Physiology. Gastrointestinal and Liver Physiology·Elena F VerdúStephen M Collins
Jan 25, 2000·American Journal of Physiology. Lung Cellular and Molecular Physiology·D J RombergerT A Wyatt

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