Induction of epigenetic variation in Arabidopsis by over-expression of DNA METHYLTRANSFERASE1 (MET1)

PloS One
Samuel BrocklehurstPeter Meyer

Abstract

Epigenetic marks such as DNA methylation and histone modification can vary among plant accessions creating epi-alleles with different levels of expression competence. Mutations in epigenetic pathway functions are powerful tools to induce epigenetic variation. As an alternative approach, we investigated the potential of over-expressing an epigenetic function, using DNA METHYLTRANSFERASE1 (MET1) for proof-of-concept. In Arabidopsis thaliana, MET1 controls maintenance of cytosine methylation at symmetrical CG positions. At some loci, which contain dense DNA methylation in CG- and non-CG context, loss of MET1 causes joint loss of all cytosines methylation marks. We find that over-expression of both catalytically active and inactive versions of MET1 stochastically generates new epi-alleles at loci encoding transposable elements, non-coding RNAs and proteins, which results for most loci in an increase in expression. Individual transformants share some common phenotypes and genes with altered gene expression. Altered expression states can be transmitted to the next generation, which does not require the continuous presence of the MET1 transgene. Long-term stability and epigenetic features differ for individual loci. Our data show that...Continue Reading

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Citations

Aug 25, 2019·International Journal of Molecular Sciences·Jin-Hong Kim
Jan 28, 2021·Physiologia Plantarum·Zhipeng GaoQiang Wei
Jan 16, 2021·International Journal of Molecular Sciences·Hymavathi SalavaFatemeh Maghuly
Mar 30, 2021·Plant Signaling & Behavior·Liu HongLiwen Jiang
Aug 11, 2021·Plants·Camille SalaünBertrand Dubreucq

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Datasets Mentioned

BETA
PRJNA395995

Methods Mentioned

BETA
PCR
transgenic
acetylation

Software Mentioned

CyMATE
STAR
R package rsubRead
Fluidigm Gene Expression Analysis
BioEdit
R package DeSeq2

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