Induction of epithelial-mesenchymal transition and down-regulation of miR-200c and miR-141 in oxaliplatin-resistant colorectal cancer cells

Biological & Pharmaceutical Bulletin
Shota TanakaSeigo Iwakawa

Abstract

Epithelial-mesenchymal transition (EMT) and changes in the expression of the microRNA-200 (miR-200) family were examined in the human colorectal cancer (CRC) cell line SW620 with acquired oxaliplatin (L-OHP) resistance. Two CRC cell lines, SW480, derived from primary CRC, and SW620, derived from lymph node metastasis, which were obtained from the same patient, were used in the present study. L-OHP-resistant SW620 cells were obtained by exposure to L-OHP for 155 d. The concentration of L-OHP was increased to 80 µM in a stepwise manner. The IC50 value of L-OHP was increased 16-fold in L-OHP-resistant SW620 cells, which also displayed mesenchymal cell-like characteristics, such as the down-regulation of E-cadherin and up-regulation of vimentin. However, L-OHP-resistant SW480 cells were not obtained when the concentration of L-OHP was increased in a similar stepwise manner. The expression levels of members of the miR-200 family (miR-200a, miR-200b, miR-429, miR-200c, and miR-141) were significantly higher in SW480 cells than in SW620 cells. The expression levels of miR-200c and miR-141 were significantly lower in L-OHP-resistant SW620 cells than in control SW620 cells. L-OHP-resistant SW620 cells did not exhibit cross-resistance to...Continue Reading

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Citations

Sep 2, 2016·Biological & Pharmaceutical Bulletin·Soyeun ParkChul-Ho Jeong
Sep 16, 2016·International Journal of Cancer. Journal International Du Cancer·Melanie MaierthalerBarbara Burwinkel
May 26, 2017·Biological & Pharmaceutical Bulletin·Shota TanakaSeigo Iwakawa
Feb 2, 2018·International Journal of Cancer. Journal International Du Cancer·Stephen J O'BrienSusan Galandiuk
Mar 3, 2017·Disease Models & Mechanisms·Ashlee M Strubberg, Blair B Madison
Oct 6, 2018·The Anatomical Record : Advances in Integrative Anatomy and Evolutionary Biology·Ming FangYanyun Li
Feb 28, 2019·BioDrugs : Clinical Immunotherapeutics, Biopharmaceuticals and Gene Therapy·Wendy W WestonH Thomas Temple
Feb 10, 2019·Journal of Personalized Medicine·Stergios BoussiosNicholas Pavlidis
Dec 9, 2017·Journal of Gastrointestinal Cancer·Fatemeh Karimi Dermani, Rezvan Najafi
Nov 13, 2016·Biomolecular Concepts·Daniel SenfterRobert M Mader
Aug 30, 2016·World Journal of Gastroenterology : WJG·Simona GurzuIoan Jung
Jun 3, 2017·BioMed Research International·María Antonia LizarbeJavier Turnay
Jul 25, 2018·World Journal of Gastroenterology : WJG·Kenneth Kw ToWilliam Cs Cho
Jun 10, 2016·International Journal of Oncology·Jing LiJian Zhang
Sep 15, 2020·Therapeutic Advances in Medical Oncology·Lili DuanDaiming Fan
Apr 24, 2018·Biomedicine & Pharmacotherapy = Biomédecine & Pharmacothérapie·Abbas Pirpour TazehkandNasser Samadi

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