PMID: 2107827Mar 1, 1990Paper

Induction of experimental chronic Pseudomonas aeruginosa lung infection with P. aeruginosa entrapped in alginate microspheres

APMIS : Acta Pathologica, Microbiologica, Et Immunologica Scandinavica
S S PedersenG N Hansen

Abstract

Alginate-producing, mucoid P. aeruginosa is frequently found in the lungs of patients with cystic fibrosis (CF), where it causes a chronic infection. The importance of alginate in the pathogenesis was demonstrated by the ability to establish chronic P. aeruginosa lung infection in rats if P. aeruginosa entrapped in minute alginate-beads were inoculated transtracheally. Alginate beads containing P. aeruginosa were formed by nebulizing a suspension of seaweed sodium-alginate and P. aeruginosa into a calcium solution. The alginate bead method of establishing infection was compared to an agar-bead method and proved to be quantitatively similar after 4 weeks. The ability of the two methods to induce formation of precipitins, IgA and IgG antibodies against P. aeruginosa antigens, including outer membrane proteins, flagella, exoenzymes and alginate, was assessed by crossed immunoelectrophoresis, enzyme-linked immunosorbent assay and immunoblotting. The two methods of inducing infection were comparable and infected rats had significantly higher antibody response than rats inoculated with sterile beads. We suggest that the alginate bead model closely resembles the later stages of CF-lung infection and that it offers the theoretical adva...Continue Reading

References

Mar 1, 1979·The Journal of Infectious Diseases·T J MarrieJ W Costerton
Jan 1, 1986·Annual Review of Microbiology·N HøibyP O Schiøtz
Jan 1, 1988·Journal of General Microbiology·J A SimpsonR T Dean
Jan 1, 1987·Annual Review of Microbiology·J W CostertonT J Marrie
Nov 1, 1983·Reviews of Infectious Diseases·J R GovanN R Baker
Aug 1, 1980·Journal of General Microbiology·J A Fyfe, J R Govan

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Citations

Jul 5, 2003·Journal of Microbiological Methods·Irma A J M Bakker-Woudenberg
Oct 7, 2004·Journal of Cystic Fibrosis : Official Journal of the European Cystic Fibrosis Society·Zhijun SongNiels Høiby
Feb 6, 2010·Nature Protocols·Thomas BjarnsholtMichael Givskov
Sep 17, 2002·The Journal of Alternative and Complementary Medicine : Research on Paradigm, Practice, and Policy·Zhijun SongArsalan Kharazmi
Dec 1, 1991·APMIS : Acta Pathologica, Microbiologica, Et Immunologica Scandinavica·H K JohansenS S Pedersen
Jan 1, 1992·APMIS : Acta Pathologica, Microbiologica, Et Immunologica Scandinavica·H K Johansen, N Høiby
Mar 1, 1993·APMIS : Acta Pathologica, Microbiologica, Et Immunologica Scandinavica·H K JohansenN Høiby
Jul 1, 1994·APMIS : Acta Pathologica, Microbiologica, Et Immunologica Scandinavica·H K JohansenN Høiby
May 1, 1996·APMIS : Acta Pathologica, Microbiologica, Et Immunologica Scandinavica·Z SongN Høiby
Jun 1, 1997·APMIS : Acta Pathologica, Microbiologica, Et Immunologica Scandinavica·Z J SongN Høiby
Dec 11, 1997·APMIS : Acta Pathologica, Microbiologica, Et Immunologica Scandinavica·C MoserN Høiby
Jun 15, 2011·Antimicrobial Agents and Chemotherapy·Wang HengzhuangNiels Høiby
Oct 24, 2012·Antimicrobial Agents and Chemotherapy·Wang HengzhuangNiels Høiby
Feb 23, 2012·Antimicrobial Agents and Chemotherapy·Wang HengzhuangNiels Høiby
Mar 24, 2005·Infection and Immunity·Nadine HoffmannNiels Høiby
Jan 5, 2005·Journal of Bacteriology·Lauren M MashburnMarvin Whiteley
Oct 1, 1996·American Journal of Respiratory and Critical Care Medicine·S de BentzmannE Puchelle
Nov 9, 2011·Respiratory Research·Antje MunderBurkhard Tümmler
Sep 26, 2013·International Journal of Molecular Sciences·Evelina PapaioannouWim J Quax
Jan 1, 2013·Pathogens·David LebeauxChristophe Beloin
Feb 26, 2009·APMIS : Acta Pathologica, Microbiologica, Et Immunologica Scandinavica·Claus MoserNiels Høiby
Dec 5, 2008·Molecular Microbiology·Yu-Sing Tammy BohnLutz Wiehlmann
May 3, 2013·APMIS. Supplementum·Thomas Bjarnsholt
Feb 27, 2013·Wound Repair and Regeneration : Official Publication of the Wound Healing Society [and] the European Tissue Repair Society·Hannah TrøstrupClaus Moser
Mar 25, 2016·APMIS : Acta Pathologica, Microbiologica, Et Immunologica Scandinavica·Dina Silke Malling DamlundClaus Moser
Sep 1, 2015·Journal of Molecular Biology·Morten RybtkeTim Tolker-Nielsen
Sep 8, 2015·Journal of Molecular Biology·Aled E L RobertsStephen P Diggle
Mar 1, 2006·International Journal of Medical Microbiology : IJMM·Thomas B Rasmussen, Michael Givskov
May 1, 2012·Microbes and Infection·Uliana KomorSusanne Häussler
Jul 6, 2013·Trends in Microbiology·Thomas BjarnsholtNiels Høiby
Aug 3, 2013·Biomicrofluidics·M SkolimowskiJ Emnéus
Dec 10, 2017·European Journal of Clinical Microbiology & Infectious Diseases : Official Publication of the European Society of Clinical Microbiology·David B HuangMark H Wilcox
Mar 5, 2017·Applied and Environmental Microbiology·Majken SønderholmThomas Bjarnsholt
Feb 3, 2016·Antimicrobial Agents and Chemotherapy·Wang HengzhuangNiels Høiby

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