Induction of fiber alignment and mechanical anisotropy in tissue engineered menisci with mechanical anchoring

Journal of Biomechanics
Jennifer L PuetzerLawrence J Bonassar

Abstract

This study investigated the effect of mechanical anchoring on the development of fiber organization and anisotropy in anatomically shaped tissue engineered menisci. Bovine meniscal fibrochondrocytes were mixed with collagen and injected into molds designed to produce meniscus implants with 12 mm extensions at each horn. After a day of static culture, 10 and 20mg/ml collagen menisci were either clamped or unclamped and cultured for up to 8 weeks. Clamped menisci were anchored in culture trays throughout culture to mimic the native meniscus horn attachment sites, restrict contraction circumferentially, and encourage circumferential alignment. Clamped menisci retained their size and shape, and by 8 weeks developed circumferential and radial fiber organization that resembled native meniscus. Clamping also increased collagen accumulation and improved mechanical properties compared to unclamped menisci. Enhanced organization in clamped menisci was further reflected in the development of anisotropic tensile properties, with 2-3 fold higher circumferential moduli compared to radial moduli, a similar ratio to native meniscus. Ten and 20mg/ml clamped menisci had similar levels of organization, with 20mg/ml menisci producing larger diamet...Continue Reading

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Citations

Apr 26, 2015·Journal of Biomechanics·Tammy L Haut DonahueSuzanne A Maher
May 6, 2015·Journal of Biomechanics·Tammy Donahue
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Dec 12, 2018·Advanced Healthcare Materials·Leanne E IannucciLawrence J Bonassar
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Feb 20, 2021·ACS Biomaterials Science & Engineering·Jongkil KimLawrence J Bonassar
Nov 13, 2017·Acta Biomaterialia·Jared L ZitnayJeffrey A Weiss
Jun 3, 2021·Journal of Biomaterials Applications·Sepideh SaghatiLeila Roshangar
Oct 10, 2016·ACS Biomaterials Science & Engineering·Stephanie RheeLawrence J Bonassar
Nov 18, 2021·Advanced Science·Claire BomkampElizabeth A Specht

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