Induction of fiber-like aggregation and gelation of collagen by ultraviolet irradiation at low temperature

International Journal of Biological Macromolecules
Chengzhi XuHaibo Wang

Abstract

Ultraviolet (UV) irradiation is a common method of molecular crosslinking and sterilization of collagen. In this study, UV irradiation at low temperature conditions was investigated as an induction and regulation method for fiber-like aggregation and gelation of collagen. Differences between gelation processes induced by UV irradiation and by traditional temperature-induced methods as well as differences in the properties of the gelation products were systematically analyzed. We found that UV irradiation can induce fiber-like aggregation and gelation of bovine tendon collagen at lower temperatures (<17 °C) than the usual temperature of 37 °C. During UV irradiation, cross-linking and degradation of collagen molecules occurred along with typical collagen fiber formation. The collagen fibers, together with the grafted collagen molecules and the degraded collagen peptides, formed a gel product that had a unique, multi-layered network structure. Collagen gels induced by UV irradiation at low temperatures displayed improved thermal stability, mechanical strength, and cell-growth promoting ability compared with collagen gels that were induced at 37 °C. Our results open up new avenues for the production of collagen-based biomaterials.

References

Mar 23, 1971·Biochimica Et Biophysica Acta·T MiyataK H Stenzel
Jul 9, 2005·Nature Materials·Scott J Hollister
Oct 13, 2005·Journal of Photochemistry and Photobiology. B, Biology·Alina Sionkowska
Sep 12, 2006·The Journal of Biological Chemistry·Karunakar KarBarbara Brodsky
Nov 8, 2007·Journal of the American Chemical Society·Shyam ReleElliot L Chaikof
Apr 7, 2009·Annual Review of Biochemistry·Matthew D Shoulders, Ronald T Raines
Dec 1, 2008·Materials Science & Engineering. C, Materials for Biological Applications·Olena S RabotyagovaDavid L Kaplan
Apr 18, 2014·Biophysical Journal·Jieling Zhu, Laura J Kaufman
Aug 13, 2015·Journal of Biomedical Materials Research. Part a·Marta MadaghieleAlessandro Sannino
Feb 4, 2016·Materials Science & Engineering. C, Materials for Biological Applications·Roman A Perez, Gemma Mestres
Jan 9, 2018·International Journal of Biological Macromolecules·Jie NanYuling Xu
Jul 26, 2018·Journal of Agricultural and Food Chemistry·Benmei WeiDong Xie
Aug 21, 2018·Advanced Materials·Anna SorushanovaDimitrios I Zeugolis
Feb 4, 2019·International Journal of Biological Macromolecules·Juntao ZhangHaibo Wang
May 14, 2018·Journal of Materials Chemistry. B, Materials for Biology and Medicine·Shichen ZhuYang Hu

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Citations

Jun 2, 2020·Journal of the Science of Food and Agriculture·Linfan ShiWuyin Weng
Oct 20, 2020·Macromolecular Bioscience·Jia Min LeeWai Yee Yeong

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