Induction of fibronectin by HER2 overexpression triggers adhesion and invasion of breast cancer cells

Experimental Cell Research
Myeongjin JeonSangmin Kim

Abstract

Fibronectin (FN), an extracellular matrix ligand, plays a pivotal role in cell adhesion, migration, and oncogenic transformation. Aberrant FN expression is associated with poor prognoses in various types of cancer, including breast cancer. In the current study, we investigated the relationship between FN induction and HER2 expression in breast cancer cells. Our results showed that the level of FN expression increased in response to HER family ligands, EGF and TGF-α in a time- and dose-dependent manner. On the other hand, EGF-induced FN expression decreased in response to trastuzumab, which is a HER2-targeted monoclonal antibody. However, EGF-induced FN expression was not affected by trastuzumab in JIMT-1 breast cancer cells, which are trastuzumab insensitive cells. Next, we introduced the HER2 gene into MDA-MB231 cells to verify the relationship between FN and HER2. The level of FN expression significantly increased in HER2-overexpressed MDA-MB231 cells. In contrast, the induction of FN by HER2 was significantly decreased in response to trastuzumab treatment. In addition, the induction of FN by HER2 was down-regulated by the MEK 1/2 specific inhibitor, U0126. Using conditioned culture media of vec- and HER2-overexpressed MDA-MB...Continue Reading

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Citations

Dec 5, 2015·Frontiers in Pharmacology·Aline Appert-CollinAmar Bennasroune
Mar 18, 2016·Tumour Biology : the Journal of the International Society for Oncodevelopmental Biology and Medicine·Sangmin KimJeong Eon Lee
May 14, 2016·Molecular and Cellular Endocrinology·Marialuisa SponzielloStefania Bulotta
Jul 25, 2018·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·Liqing LiCai Huang
Dec 6, 2016·Acta Histochemica Et Cytochemica·Eriko MurakamiNorimichi Nemoto
Sep 7, 2017·Cancer Immunology, Immunotherapy : CII·Mohammad W KhanKhashayarsha Khazaie
Feb 11, 2020·British Journal of Cancer·Hege F BergHenrica M J Werner
Mar 28, 2021·Cancer Letters·Hyeong-Jun HanYong-Nyun Kim

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