Induction of gametogenesis in the basal cnidarian Nematostella vectensis(Anthozoa)

Development Genes and Evolution
Jens H Fritzenwanker, Ulrich Technau

Abstract

A protocol was established to reproducibly induce spawning in the basal cnidarian Nematostella vectensis (Anthozoa). We found that a combination of feeding regime, dark-light cycle and temperature shift synergistically induced gametogenesis in adult polyps. Females lay between 100-600 eggs. This procedure led reproducibly to the production of thousands of eggs over the course of more than 1 year in weekly cycles. Gametes are released in a time window of about 2 h resulting in predictable and fairly synchronized development. We also present a method for in vitro fertilization allowing manipulation of early embryos. These methods as well as the simple culture conditions could provide important prerequisites for the use of Nematostella as a model system for the development of a basal Metazoa.

Citations

Sep 30, 2005·Development Genes and Evolution·Craig R MagieMark Q Martindale
Nov 26, 2003·Current Opinion in Genetics & Development·Nina M Brooke, Peter W H Holland
Jul 6, 2012·Nature·Patrick R H SteinmetzUlrich Technau
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Mar 11, 2011·Development·Ulrich Technau, Robert E Steele
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Dec 1, 2012·Toxicology Mechanisms and Methods·Alfredo Ambrosone, Claudia Tortiglione
Dec 6, 2014·Developmental Biology·Chiara SinigagliaFabian Rentzsch

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