PMID: 3749104Jan 1, 1986Paper

Induction of gene mutations in mice: the multiple endpoint approach

Progress in Clinical and Biological Research
U H Ehling

Abstract

The multiple endpoint mammalian mutagenesis approach developed in our institute screens in the same animal for recessive specific locus alleles at 7 loci, approximately 30 loci coding for dominant cataract mutations, 23 loci controlling protein-charge changes and 12 loci for enzyme-activity alterations. Experiments to screen for the approximately 70 loci in the same offspring of treated male mice were performed with ethylnitrosourea (ENU), procarbazine and X-rays. Mutations were recovered for each genetic endpoint in all treatment groups where a sufficient number of offspring was scored. The observed per locus mutation rate for the different genetic endpoints after spermatogonial treatment with 250 mg/kg ENU was 93.6 X 10(-5) for specific locus mutations, 66.0 X 10(-5) for enzyme-activity mutations, 6.1 X 10(-5) for dominant cataract mutations, and 3.1 X 10(-5) for protein-charge mutations. In all experiments the mutation rates to specific locus and enzyme-activity alleles were higher than the mutation rates to either dominant cataract or protein-charge alleles. The mutations were confirmed by breeding tests.

Citations

Feb 5, 1999·Progress in Retinal and Eye Research·J Graw
Aug 1, 1991·Mutation Research·J Peters, S E Lewis
Apr 26, 2003·Mutation Research·Carles Vilariño-GüellYuri E Dubrova

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