Induction of glial glutamate transporters in adult mesenchymal stem cells

Journal of Neurochemistry
Isabelle de HemptinneEmmanuel Hermans

Abstract

Adult bone marrow mesenchymal stem cells are multipotent cells that can differentiate into a variety of mesodermal tissues. Recent studies have reported on their ability to also evolve into non-mesodermal cells, especially neural cells. While most of these studies revealed that manipulating these cells triggers the expression of typical neurone markers, less is known about the induction of neuronal- or glial-related physiological properties. The present study focused on the characterisation of glutamate transporters expression and activity in rat mesenchymal stem cells grown in culture conditions favouring their differentiation into astroglial cells. Ten days exposure of the cells to the culture supplement G5 was found to increase the expression of nestin (neuro-epithelial stem cell intermediate filament), an intermediate filament protein expressed by neural stem cells. Simultaneously, a robust induction of the high-affinity glutamate transporter GLT-1 (and GLAST) expression was detected by RT-PCR and immunocytochemistry. This expression was correlated with a highly significant increase in the Na+-dependent [3H]D-aspartate uptake. Finally, while glial fibrillary acidic protein immunoreactivity could not be detected, the induced...Continue Reading

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Citations

Jul 8, 2010·Langmuir : the ACS Journal of Surfaces and Colloids·Sumit MehrotraChristina Chan
Mar 24, 2010·Journal of Visualized Experiments : JoVE·Linxia Zhang, Christina Chan
Oct 14, 2009·Expert Opinion on Biological Therapy·Ofer SadanDaniel Offen
Apr 28, 2009·Differentiation; Research in Biological Diversity·Florian VöllnerChristian Morsczeck
May 12, 2009·Neurochemistry International·Sébastien TilleuxEmmanuel Hermans
Jan 22, 2008·Best Practice & Research. Clinical Gastroenterology·Robert Jaster, Jörg Emmrich
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Dec 30, 2008·Journal of Biochemical and Molecular Toxicology·Hong DanYing-Hui Liu
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Nov 23, 2006·Biochemical and Biophysical Research Communications·Claus KordesDieter Häussinger
Jun 14, 2020·Scientific Reports·Karl F SchüttlerJürgen R J Paletta

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