PMID: 8597588Feb 16, 1996Paper

Induction of glucosylceramide synthase by synthase inhibitors and ceramide

Biochimica Et Biophysica Acta
A AbeJ A Shayman

Abstract

Glucosylceramide (GlcCer) synthase acts on the sphingolipid, ceramide, to transer a glucose moiety from UDP-glc, thus forming the first member of a large family of glucosphingolipids. Two inhibitors of the enzyme, D-threo-1-phenyl-2-decanoylamino-3-morpholino-1-propanol (D-threo-PDMP) and N-butyldeoxynojirimycin (NBDN), have been found to induce an elevated level of the synthase in MDCK cells. In cells treated with 20 muM PDMP, then assayed for synthase activity under conditions in which the absorbed PDMP was partially diluted out, the assay showed that the enzyme's specific activity had risen considerably in only 1 h and reached a maximum of about three times the control activity within 6 h. Both cycloheximide and actinomycin D, inhibitors of translational and transcriptional protein synthesis, caused much of the synthase activity to disappear in 6 h, presumably because of normal catabolic destruction. However, simultaneous inclusion of PDMP or NBDN in the cell medium slowed the rate of synthase disappearance. L-Cycloserine, which blocked the synthesis of ceramide, nevertheless allowed PDMP to elevate the synthase activity. Thus the inductive effect appears to be due, in part at least, to resistance of the enzyme-inhibitor com...Continue Reading

References

Feb 1, 1992·Journal of Biochemistry·A AbeN S Radin
Jan 1, 1991·Enzyme·N S Radin, G S Shukla
May 15, 1990·Archives of Biochemistry and Biophysics·B K GillardD M Marcus
Dec 1, 1990·Archives of Biochemistry and Biophysics·G S Shukla, N S Radin
Aug 15, 1990·Archives of Biochemistry and Biophysics·K FurukawaK O Lloyd
Aug 1, 1988·Biochemical Pharmacology·N S Radin, J Inokuchi
Jan 1, 1987·Annual Review of Biochemistry·A D Elbein
Aug 5, 1966·Journal of the American Chemical Society·R C Gaver, C C Sweeley
Apr 1, 1980·Chemistry and Physics of Lipids·R R Vunnam, N S Radin
Apr 28, 1995·Biochimica Et Biophysica Acta·N D Ridgway, D L Merriam

❮ Previous
Next ❯

Citations

May 24, 2005·Biochimica Et Biophysica Acta·Ryan J Perry, Neale D Ridgway
Jun 14, 2003·Philosophical Transactions of the Royal Society of London. Series B, Biological Sciences·Terry D ButtersFrances M Platt
Apr 14, 2011·Journal of Lipid Research·Takuji NabetaniYoshio Hirabayashi
Nov 20, 2015·Biochimie·Leonardo AstudilloThierry Levade
Nov 10, 2006·Drug Resistance Updates : Reviews and Commentaries in Antimicrobial and Anticancer Chemotherapy·K E Rizzieri, Y A Hannun
May 4, 2011·Journal of Surgical Oncology·Xiaofang ZhangGengyin Zhou
Jun 8, 2004·Journal of Cerebral Blood Flow and Metabolism : Official Journal of the International Society of Cerebral Blood Flow and Metabolism·Kenzo TakahashiJohn M Hallenbeck
Aug 1, 1997·Thyroid : Official Journal of the American Thyroid Association·A E PekaryJ M Hershman
Feb 5, 2008·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·Yong-Yu LiuMyles C Cabot
Sep 21, 2001·The Journal of Immunology : Official Journal of the American Association of Immunologists·T KantoA A Amoscato
Oct 3, 1998·The Journal of Biological Chemistry·H S OverkleeftJ M Aerts
Jan 27, 1999·Biochemical and Biophysical Research Communications·S IchikawaY Hirabayashi
Aug 8, 1998·Trends in Cell Biology·S Ichikawa, Y Hirabayashi
Jul 9, 1999·Biochimica Et Biophysica Acta·R GhidoniA Giuliani
Jan 18, 2003·Biochimica Et Biophysica Acta·Richard J Bleicher, Myles C Cabot
Mar 2, 2011·Langmuir : the ACS Journal of Surfaces and Colloids·Fernando DupuyBruno Maggio
Jan 24, 2017·Blood Cells, Molecules & Diseases·Ebru CandaMahmut Coker

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.