Induction of Gonadal Development in Protogynous Grouper with Orally Delivered FSH DNA

Marine Biotechnology
Peter PalmaAbigail Elizur

Abstract

The availability of sexually mature fish often dictates the success of its captive breeding. In this study, we induced reproductive development in juvenile protogynous tiger grouper through oral administration of a plasmid (p) containing an engineered follicle-stimulating hormone (FSH). An expression construct (pcDNA3.1) was designed to express a single-chain FSH consisting of giant grouper FSH β-subunit and glycoprotein subunit-α (CGα), linked by the carboxy-terminal peptide (CTP) sequence from the human chorionic gonadotropin (hCG). Single oral delivery of pFSH encapsulated in liposome and chitosan to tiger grouper yielded a significant increase in plasma FSH protein level after 4 days. Weekly pFSH feeding of juvenile tiger groupers for 8 weeks stimulated ovarian development as indicated by a significant increase in oocyte diameter and progression of oocytes to cortical alveolar stage. As the pFSH treatment progressed from 20 to 38 weeks, female to male sex change was initiated, characterized by oocyte regression, proliferation of spermatogonial cells, and occurrence of spermatogenic cysts. It was also associated with significantly lower mRNA expression of steroidogenic genes (cyp11b, cyp19a1a, and foxl2) and basal plasma lev...Continue Reading

References

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Mar 12, 2016·General and Comparative Endocrinology·Pablo J Sanchis-BenllochAbigail Elizur

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Citations

Jul 21, 2020·General and Comparative Endocrinology·G MolésA Gómez
Jul 3, 2020·General and Comparative Endocrinology·Yonathan Zohar

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Methods Mentioned

BETA
ELISA
electrophoresis
PCR
transfection

Software Mentioned

ImageJ

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