Abstract
The availability of sexually mature fish often dictates the success of its captive breeding. In this study, we induced reproductive development in juvenile protogynous tiger grouper through oral administration of a plasmid (p) containing an engineered follicle-stimulating hormone (FSH). An expression construct (pcDNA3.1) was designed to express a single-chain FSH consisting of giant grouper FSH β-subunit and glycoprotein subunit-α (CGα), linked by the carboxy-terminal peptide (CTP) sequence from the human chorionic gonadotropin (hCG). Single oral delivery of pFSH encapsulated in liposome and chitosan to tiger grouper yielded a significant increase in plasma FSH protein level after 4 days. Weekly pFSH feeding of juvenile tiger groupers for 8 weeks stimulated ovarian development as indicated by a significant increase in oocyte diameter and progression of oocytes to cortical alveolar stage. As the pFSH treatment progressed from 20 to 38 weeks, female to male sex change was initiated, characterized by oocyte regression, proliferation of spermatogonial cells, and occurrence of spermatogenic cysts. It was also associated with significantly lower mRNA expression of steroidogenic genes (cyp11b, cyp19a1a, and foxl2) and basal plasma lev...Continue Reading
References
Feb 1, 1976·FEBS Letters·H M Patel, B E Ryman
Jun 1, 1996·Pharmaceutical Research·H TakeuchiY Kawashima
Jan 11, 2000·Comparative Biochemistry and Physiology. Part B, Biochemistry & Molecular Biology·M D RahmanK Takano
Mar 10, 2005·Marine Biotechnology·Erwin A RamosCelia Aurora T Torres-Villanueva
Jul 15, 2005·Biology of Reproduction·Xiao HuangRongjia Zhou
Jun 7, 2006·Journal of Drug Targeting·Jringjai ThongborisuteHirofumi Takeuchi
Nov 10, 2006·Molecular and Cellular Endocrinology·Wei XiaJian-Fang Gui
Jan 24, 2007·Journal of Molecular Endocrinology·Hyeyeon KoYoung Chang Sohn
May 3, 2008·General and Comparative Endocrinology·Mohammad Ashraful AlamMasaru Nakamura
May 16, 2008·Fish & Shellfish Immunology·S Rajesh KumarA S Sahul Hameed
Aug 8, 2008·Biology of Reproduction·Yukinori KazetoKohei Yamauchi
Oct 25, 2008·Endocrinology·Yasuhisa KobayashiYoshitaka Nagahama
Mar 26, 2009·General and Comparative Endocrinology·Constantinos C MylonasSilvia Zanuy
Apr 28, 2009·General and Comparative Endocrinology·Yonathan ZoharOlivier Kah
Aug 19, 2009·General and Comparative Endocrinology·B Levavi-SivanJ J Lareyre
Feb 12, 2010·Biology of Reproduction·Yasuhisa KobayashiMasaru Nakamura
Jun 12, 2012·General and Comparative Endocrinology·Jun ChenWensheng Li
Oct 15, 2013·Journal of Medical Virology·Jing LiuBeixing Liu
Nov 22, 2013·Biology of Reproduction·María José MazónSilvia Zanuy
Nov 19, 2015·Scientific Reports·Qing HuDapeng Li
Mar 12, 2016·General and Comparative Endocrinology·Pablo J Sanchis-BenllochAbigail Elizur
Feb 10, 2017·PloS One·Huan WangShaoxiong Ding
Oct 11, 2017·PloS One·Guan-Chung WuChing-Fong Chang
Jul 17, 2018·Biology of Reproduction·Yulong HanHaoran Lin
Oct 30, 2018·Biology of Reproduction·Peter PalmaAbigail Elizur
Jun 22, 2019·General and Comparative Endocrinology·Josephine NocilladoAbigail Elizur
Citations
Nov 1, 2020·Molecular and Cellular Endocrinology·Guan-Chung WuChing-Fong Chang
Jul 21, 2020·General and Comparative Endocrinology·G MolésA Gómez
Jul 3, 2020·General and Comparative Endocrinology·Yonathan Zohar
Aug 19, 2021·The CRISPR Journal·Adi Segev-HadarJakob Biran