PMID: 9425169Feb 14, 1998Paper

Induction of growth cone formation by transient and localized increases of intracellular proteolytic activity

The Journal of Cell Biology
N E Ziv, M E Spira

Abstract

The formation of a growth cone at the tip of a transected axon is a crucial step in the subsequent regeneration of the amputated axon. During this process, the transected axon is transformed from a static segment into a motile growth cone. Despite the importance of this process for regeneration of the severed axon, little is known about the mechanisms underlying this transformation. Recent studies have suggested that Ca2+-activated proteinases underlay the morphological remodeling of neurons after injury. However, this hypothesis was never tested directly. Here we tested the ability of transient and localized increases in intracellular proteolytic activity to induce growth cone formation and neuritogenesis. Minute amounts of the proteinase trypsin were microinjected into intact axonal segments or somata of cultured Aplysia neurons, transiently elevating the intracellular protease concentration to 13-130 nM in the vicinity of the injection site. Such microinjections were followed by the formation of ectopic growth cones and irreversible neuritogenesis. Growth cones were not formed after external application of trypsin, microinjection of the carrier solution, or inactivated trypsin. Growth cone formation was not preceded by incre...Continue Reading

References

Oct 1, 1978·Proceedings of the National Academy of Sciences of the United States of America·D BrayG Shaw
Jan 1, 1977·Experimental Cell Research·G Shaw, D Bray
Dec 1, 1978·Experimental Cell Research·N K WessellsR P Nuttall
Aug 1, 1992·BioEssays : News and Reviews in Molecular, Cellular and Developmental Biology·D E GollT Zalewska
Dec 1, 1989·Trends in Neurosciences·D J Goldberg, D W Burmeister
Aug 1, 1987·Proceedings of the National Academy of Sciences of the United States of America·P W BaasS R Heidemann
Apr 2, 1987·Nature·R D Burgoyne, T R Cheek
Jan 1, 1987·Experimental Brain Research·D G EmeryG W Gross
Jan 1, 1987·Experimental Brain Research·G W Gross, M L Higgins
Sep 1, 1986·The Journal of Cell Biology·P W Baas, S R Heidemann
Mar 1, 1986·Experimental Cell Research·H C JoshiS R Heidemann
Jun 8, 1984·Science·G Lynch, M Baudry
Jun 1, 1984·Proceedings of the National Academy of Sciences of the United States of America·R SimanG Lynch
Oct 1, 1995·Physiological Reviews·R B Maccioni, V Cambiazo
Oct 1, 1994·Current Opinion in Neurobiology·C H LinP Forscher
Nov 1, 1994·Trends in Pharmacological Sciences·K K Wang, P W Yuen
Feb 1, 1994·Current Opinion in Cell Biology·N Hirokawa
Feb 1, 1994·Current Opinion in Neurobiology·D Bentley, T P O'Connor
Jan 1, 1993·Annual Review of Cell Biology·V Bennett, D M Gilligan
Jun 1, 1993·The Journal of Cell Biology·C H Lin, P Forscher
Apr 16, 1996·Proceedings of the National Academy of Sciences of the United States of America·J W HellW A Catterall
Apr 29, 1997·Proceedings of the National Academy of Sciences of the United States of America·C M GodellG D Bittner

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Citations

Feb 2, 2000·BioEssays : News and Reviews in Molecular, Cellular and Developmental Biology·S S Andersen, G Q Bi
Jun 20, 2003·Journal of Neuroscience Methods·I SahlyMicha E Spira
Jan 14, 2005·The Journal of Neuroscience : the Official Journal of the Society for Neuroscience·Poonam VermaJames W Fawcett
May 19, 2009·Neuroscience Research·Mario PellegrinoFrancesca De Gregorio
Sep 30, 2003·Journal of Neuroscience Research·Matthew S Geddis, Vincent Rehder
May 11, 2011·Developmental Neurobiology·Michael Stiess, Frank Bradke
Apr 9, 2009·Journal of Biomedical Materials Research. Part a·Jae Young LeeChristine E Schmidt
Oct 29, 2005·Journal of Neuroscience Methods·Wesley C ChangDavid W Sretavan
Feb 13, 2001·Experimental Neurology·I Sellés-NavarroL McKerracher
Aug 18, 2010·Communicative & Integrative Biology·Elizabeth Tuck, Valeria Cavalli
Sep 23, 2014·Neuroscience·A DeFrancesco-LisowitzR E Zigmond
Aug 25, 2009·Experimental Neurology·Ida Rishal, Mike Fainzilber
May 5, 2011·Molecular and Cellular Neurosciences·Ona E Bloom, Jennifer R Morgan
Aug 10, 2000·Journal of Neurobiology·G Gallo, P C Letourneau
Oct 22, 2005·Neurosurgery·David W SretavanMichel Kliot
May 2, 2001·Nature Cell Biology·P L McNeil, M Terasaki
Jul 29, 2020·Frontiers in Cellular Neuroscience·William RodemerMichael E Selzer

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