Induction of hepatic acute-phase protein transcripts: differential effects of acute and subchronic dimethylnitrosamine exposure in vivo

Toxicology and Applied Pharmacology
J F LockwoodL B Schook

Abstract

Inflammatory responses are accompanied by increased expression of hepatocyte-derived proteins collectively known as acute phase reactants (APR). B6C3F1 female mice were exposed intraperitoneally every 24 hr to either vehicle (PBS) or DMN (5 mg/kg) for up to six exposures. Following a single treatment (acute), liver tissues were collected at 3, 6, 12, and 24 hr post-exposure. The same collection scheme was repeated following the fourth and sixth exposures (subchronic). Total cellular RNA was isolated and Northern blot analyses were performed using 3'-end radiolabeled oligonucleotides specific for serum amyloid A (SAA), serum amyloid P (SAP), and albumin (ALB). SAA transcripts were detected 3 hr after acute DMN exposure, peaked at 6 hr, and rapidly declined to vehicle control levels by 12 hr. No SAA transcripts were observed in vehicle-treated controls. In contrast, SAP transcripts were constitutively expressed in both vehicle and DMN-treated groups throughout the acute exposure period. However, at 3 and 6 hr after DMN exposure, elevated levels of SAP transcripts were observed before returning to control levels at 12 and 24 hr. Expression of albumin transcripts decreased rapidly following acute DMN exposure and remained suppresse...Continue Reading

Citations

Aug 25, 2001·The British Journal of Nutrition·H CrewsG Williamson
Jul 16, 2011·Cellular Immunology·M Teresa OrtegaStephen K Chapes
Apr 25, 2008·Current Protocols in Immunology·A H Fortier, L A Falk
Jun 19, 1998·Toxicology and Applied Pharmacology·A BhattacharjeeL B Schook

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.