Induction of hepatocyte growth by intraportal infusion of HGF into beagle dogs

Biochemical and Biophysical Research Communications
Y KobayashiT Nakamura

Abstract

Hepatocyte growth factor (HGF), originally identified as a potent mitogen for mature parenchymal hepatocytes, is a hepatotrophic factor involved in liver regeneration and is even essential for development of the liver. We report here that human recombinant HGF at a very low concentration and given intraportaly stimulated liver regeneration in dogs. In vitro, HGF dose-dependently stimulated DNA synthesis of primary cultured hepatocytes isolated from a dog. The maximal activity was twofold higher than that of epidermal growth factor, and insulin potentiated the mitogenic activity of HGF. When human recombinant HGF was infused through the portal vein into 30% partially hepatectomized dogs at 0.25 microg/kg body weight in order to directly target the liver, HGF stimulated DNA synthesis of hepatocytes and liver weight at 72 h after the operation; labeling indices in saline- and HGF-injected groups were 0.75 and 1.82%, respectively, and the liver weights in saline- and HGF-injected groups were 302 and 374 g, respectively. Since HGF exerts potent antihepatitis activity as well as mitogenic activity, these results indicate that intraportal administration of HGF may be particularly important to enhance liver regeneration and prevent the...Continue Reading

Citations

Mar 19, 2009·ILAR Journal·Christine Gagliardi, Bruce A Bunnell
May 1, 2007·Regenerative Medicine·Shinya Mizuno, Toshikazu Nakamura
Aug 24, 2010·World Journal of Gastroenterology : WJG·Hussein-M Atta
Sep 17, 2002·Proceedings of the National Academy of Sciences of the United States of America·Katherine Parker PonderMark E Haskins
Dec 9, 2008·Animal Reproduction Science·Mohammad Shamim HosseinWoo Suk Hwang
Mar 4, 2008·The Veterinary Journal·Brigitte ArendsJan Rothuizen
May 18, 2000·Journal of Orthopaedic Research : Official Publication of the Orthopaedic Research Society·R FerraciniP Buracco
Dec 31, 1997·The American Journal of Physiology·K X LiuY Sugiyama
Feb 8, 2003·Acta Paediatrica. Supplement·M HaskinsU Giger

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