PMID: 2506637Sep 1, 1989Paper

Induction of high-affinity interleukin 2 receptors on human T lymphocytes. The role of calcium and protein kinase C

Scandinavian Journal of Immunology
C S Larsen, N O Christiansen

Abstract

The relationship between free cytoplasmic calcium, activation of protein kinase C (PKC) and expression of high-affinity interleukin 2 receptors (HA-IL-2R) on human T lymphocytes was studied. Induction of HA-IL-2R by phytohaemagglutinin (PHA) was associated with an increase in free cytoplasmic calcium and a transient increase in membrane-associated PKC. However, whereas addition of EGTA inhibited induction of receptors by PHA, addition of the PKC-inhibitor H7 did not. 12-o-tetradecanoyl-phorbol-13-acetate (PMA) and 1-oleoyl-2-acetyl-rac-glycerol (OAG) were both found to activate and translocate PKC. However, only PMA induced expression of HA-IL-2R. Not surprisingly, the effect of PMA was independent of extracellular calcium, but was inhibited by H7. Furthermore, a correlation between the number of HA-IL-2R and free cytoplasmic calcium upon stimulation with ionomycin was observed. Associated with the rise in intracellular calcium, the ionophore caused a slight increase in membrane-associated PKC. Also, addition of H7 inhibited expression of HA-IL-2R. Finally, OAG and ionomycin acted synergistically on expression of HA-IL-2R. In conclusion, induction of HA-IL-2R requires at least two different signals and neither activation of PKC...Continue Reading

References

Jun 15, 1987·Biochemical and Biophysical Research Communications·R B ClarkR I Sha'afi
Aug 1, 1987·Clinical Immunology and Immunopathology·A E NelR M Galbraith
Jul 18, 1986·Science·Y Nishizuka
Apr 1, 1987·Proceedings of the National Academy of Sciences of the United States of America·R J RobbW C Greene
Jun 11, 1987·Nature·I Trowbridge
Jun 1, 1987·Proceedings of the National Academy of Sciences of the United States of America·M TsudoT A Waldmann
Aug 1, 1985·European Journal of Immunology·M A ValentineD A Carson
Feb 21, 1986·Biochimica Et Biophysica Acta·N O Christiansen, H Juhl
Nov 30, 1984·Biochemical and Biophysical Research Communications·S Kawamoto, H Hidaka
Jan 1, 1983·Proceedings of the National Academy of Sciences of the United States of America·J E NiedelG R Vandenbark
Jun 1, 1983·Proceedings of the National Academy of Sciences of the United States of America·G A KoretzkyP C Nowell
Jul 1, 1984·Immunology Today·R J Robb
Mar 1, 1985·Immunology Today·A Townsend

❮ Previous
Next ❯

Citations

Jul 26, 2005·Biochemistry. Biokhimii︠a︡·S V Khaidukov, I S Litvinov
Mar 1, 1990·Scandinavian Journal of Immunology·C S Larsen
Nov 9, 2012·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·Justin H GundelachRichard J Bram

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.