Induction of hsp72 in heat-treated rat embryos: a tissue-specific response

Teratology
B R FisherK M Brown

Abstract

Previous studies have demonstrated that heat exposure on gestation day 10 (GD10) resulted in disrupted somite development in rat embryos 24 hr after exposure and in thoracic skeletal malformations in neonatal rats examined 3 days postpartum. The production of abnormal somites was correlated with the location of skeletal elements that developed from the affected somites. Heat has also been shown to induce changes in genetic expression whereby new proteins are synthesized and the expression of constituent proteins may be repressed. In the present study, heat-induced alterations in protein synthesis during rat organogenesis that may be associated with previously observed malformation was investigated. GD10 rat embryos were exposed in utero to a heat treatment previously demonstrated to produce skeletal malformations; maternal core temperature was raised and maintained at 42-42.4 degrees C for 5 min. In addition, explanted GD10 embryos were cultured in vitro and exposed to temperatures of 42-42.5 degrees C for 15 min. At various times postexposure, embryos were labeled with 35S-methionine and processed for SDS-PAGE. In both in vivo and in vitro heat-treated embryos, a transient enhanced de novo synthesis of 70- and 90-kD proteins w...Continue Reading

References

Apr 1, 1979·Teratology·A G HendrickxK Matayoshi
Feb 1, 1978·Biological Reviews of the Cambridge Philosophical Society·D A New
Apr 1, 1992·Toxicology and Applied Pharmacology·P L GoeringC A Dick
Jan 1, 1991·Teratogenesis, Carcinogenesis, and Mutagenesis·K HondaM Yukioka
Jun 1, 1990·Science·Y Sanchez, S L Lindquist
Jan 1, 1990·Advances in Genetics·N S Petersen
Jan 1, 1986·Annual Review of Biochemistry·S Lindquist
Jan 1, 1986·Teratogenesis, Carcinogenesis, and Mutagenesis·M J Edwards
Jan 1, 1985·International Journal of Hyperthermia : the Official Journal of European Society for Hyperthermic Oncology, North American Hyperthermia Group·W U MullerL S Goldstein
Sep 1, 1993·Toxicology and Applied Pharmacology·P L GoeringC L Kish
Mar 1, 1993·Teratology·C A KimmelJ Chen
Mar 1, 1993·Teratology·G L KimmelP M Silverman

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Citations

Feb 1, 1997·Developmental Dynamics : an Official Publication of the American Association of Anatomists·J M Thayer, P E Mirkes
Nov 1, 2002·The Journal of Experimental Zoology·Javier MartinezFilomena Rodriguez-Caabeiro
Apr 30, 2011·Comparative Biochemistry and Physiology. Part A, Molecular & Integrative Physiology·Weronika RupikWiesława Widłak
Jan 28, 2004·Birth Defects Research. Part B, Developmental and Reproductive Toxicology·Andriana D PapaconstantinouPeter L Goering
Aug 26, 2011·Cellular Signalling·Héctor R QuintáMario D Galigniana

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