Induction of human autologous cytotoxic T lymphocytes against minced tissues of glioblastoma multiforme

Journal of Neurosurgery
H TsurushimaT Ohno

Abstract

The authors induced autologous cytotoxic T lymphocytes (CTLs) directly from peripheral blood lymphocytes by preparing a coculture of minced tissue fragments of glioblastoma multiforme (GBM) with interleukins-1, -2, -4, and -6 and interferon-gamma in RHAM alpha medium containing 5% autologous plasma for 2 weeks. At the end of this period, the frequencies of CD3+, CD4+, CD8+, and CD16+ lymphocytes were 95% to 99%, 40% to 62%, 37% to 38%, and 0.2%, respectively. The lymphocytes killed 82% to 100% of the GBM cells within 48 hours at an effector-to-target cell ratio of 1.67, whereas in a separate coculture, autologous lymphokine-activated killer (LAK) cells killed only 33% of GBM cells under the same conditions. The lymphocytes showed no cytotoxicity against LAK-sensitive Daudi cells, natural killer-sensitive K562 cells or autologous fibroblasts grown from the brain tumor, although they did show slight cytotoxicities against allogeneic GBM cell lines. These results lead the authors to suggest that the lymphocyte population contains specific CTLs for autologous brain tumor cells and that these CTLs could be effective in adoptive immunotherapy to combat brain tumor.

References

Dec 1, 1978·Proceedings of the National Academy of Sciences of the United States of America·I HeronK Berg
Jun 1, 1979·The Journal of Experimental Medicine·S GillisK A Smith
Jun 1, 1989·The Journal of Experimental Medicine·B MukherjiM T Ergin
Apr 15, 1987·International Journal of Cancer. Journal International Du Cancer·T E RobertsM Moore
Apr 15, 1994·Cellular Immunology·M NakashimaZ Steplewski
Jan 1, 1993·Cancer Immunology, Immunotherapy : CII·T TakahashiT Yoshiki
Feb 1, 1993·Clinical Immunology and Immunopathology·C G Ioannides, T L Whiteside

❮ Previous
Next ❯

Citations

Jul 4, 2012·Neurosurgery Clinics of North America·Seunggu J HanAndrew T Parsa
Dec 1, 2009·Neurosurgery Clinics of North America·Seunggu J HanMichael Lim
Dec 16, 1998·Japanese Journal of Cancer Research : Gann·M KohyamaT Ohno
Jul 3, 1999·Japanese Journal of Cancer Research : Gann·H TsurushimaT Ohno
Jun 13, 2003·Microbiology and Immunology·Tadao Ohno
Jan 25, 2005·International Journal of Urology : Official Journal of the Japanese Urological Association·Koji KawaiHideyuki Akaza

❮ Previous
Next ❯

Methods Mentioned

BETA
flow cytometry

Related Concepts

Related Feeds

Cancer Biology: Molecular Imaging

Molecular imaging enables noninvasive imaging of key molecules that are crucial to tumor biology. Discover the latest research in molecular imaging in cancer biology in this feed.